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pEBG-NHERF2

Plasmid

#28292

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF2 (NHERF2 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pGex-3x MKK4

Plasmid

#14617

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Tags

GST

Expression

Bacterial

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pSuper mouse DGCR8-3

Plasmid

#14774

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (Dgcr8 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
p16-1 hTRF2

Plasmid

#12299

Depositor

Titia de Lange

Article

Broccoli et al Nat Genet. 1997 Oct . 17(2):231-5.

Insert

hTRF2 (TERF2 Human)

Expression

Bacterial

Mutation

A434 deleted; There is a stop codon in the 5'…

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-RNF125

Plasmid

#122045

Purpose

express RNF125

Depositor

Ze'ev Ronai

Insert

RNF125 (RNF125 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pKD227

Plasmid

#90954

Purpose

Expresses S. baltica TtrS under the Ptac promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

Tetrathionate sensor kinase

Expression

Bacterial

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-shcIAP1A

Plasmid

#44129

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

cIAP1

Use

Lentiviral and RNAi

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
pTETSIX

Plasmid

#74104

Purpose

mCherry-encoding vector for markerless deletion mutagenesis in Bradyrhizobium diazoefficiens and other Alphaproteobacteria

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

tetA(C)

Use

Conjugative bacterial vector

Mutation

C-to-T transition at pos. no. 1049 of tetA(C) lea…

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pFlp-Tc

Plasmid

#222336

Purpose

Expresses Flp and tetracycline to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sacB (sacB )
flp (flp )
cI (cI )

Promoter

cl, flp, and sacB

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE162

Plasmid

#118510

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE160

Plasmid

#118508

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAR-9.47_VP1,3

Plasmid

#74240

Purpose

Original plasmid described in Choudhury et al. 2015 Mol Ther. for production of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP1
AAV9.47 VP3

Use

AAV

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pBdox-SPI1-T2A-CEBPA-P2A-FLI1_BleoR

Plasmid

#250291

Purpose

PiggyBac integration of 3 transcription factors (SPI1, CEBPA, FLI1) under a dox-inducible promoter

Depositor

George Church

Article

Liu et al Nat Commun. 2025 Jun 10;16(1):5136. doi:

Insert

SPI1-T2A-CEBPA-P2A-FLI1

Expression

Mammalian

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

Plasmid

#205447

Purpose

human AAVS1-targeting plasmid for expression of mouse CR1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCR1

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-ATG9A

Plasmid

#198529

Purpose

Expression of ATG9A-GFP fusion protein in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

ATG9A (ATG9A Human)

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations in codons 4 and 775

Promoter

CMV

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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FHL-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129628

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

FHL-cpmTq2-Calcium-lifetime-sensor

Tags

6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial and Mammalian

Promoter

CMV,&Rhamnose

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.MTF2

Plasmid

#125169

Purpose

Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

MTF2 (MTF2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

Plasmid

#110555

Purpose

pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

Depositor

Martin Pule

Article

Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

Insert

Anti-RD114 heavy chain

Tags

mIgG2A

Expression

Bacterial and Mammalian

Promoter

EF-1 alpha core promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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iChr2-Control-Mosaic (SO274)

Plasmid

#99751

Purpose

Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HIs-H2B-Cherry, H2B-EGFP, HA-H2B-Cerulean

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-IL6.Pro

Plasmid

#79491

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Il6 Promoter (IL6 Human, Synthetic)

Use

Gateway Cloning

Promoter

IL6 Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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