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Plasmid#159275PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInserttdTomato
UseAAV; S1 knockin donor vectorExpressionMammalianPromoterCAGGSAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA
Plasmid#47457PurposeLITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS(alpha-imp)-CRY2PHR-NLS-VP64
Tags2A_GFPExpressionMammalianPromoterEF1-alphaAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNIC-CTHF
Plasmid#26105PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTagsTEV - His6 - FlagExpressionBacterialAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJR103
Plasmid#187242PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
UseLentiviralAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CAG-SPOTlight-U2GCR
Plasmid#164819PurposeReporter to measure ISR state-dependent protein synthesisDepositorInsertSPOTlight-U2GCR
UseAAVExpressionMammalianPromoterCAGAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMC-TNFalpha
Plasmid#20210DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTNFa 3' UTR (TNF Human)
ExpressionMammalianPromoterCMVAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGH153_MS2-AIDΔ-Hygro
Plasmid#85416Purposelentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable markerDepositorInsertMS2-AIDΔ and Hygromycin resistance
UseCRISPR and LentiviralExpressionMammalianAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-tetO-Eomes
Plasmid#70271PurposeLentiviral plasmid for tetracycline/doxycycline dependent expression of murine EomesDepositorInsertEomes (Eomes Mouse)
PromotertetOAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICE-FLAG-NAT10-siR-WT
Plasmid#59365PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman NAT10 (NAT10 Human)
TagsFLAGExpressionMammalianMutationMutations to render cDNA resistant to a siRNAPromoterCMV-tetAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223-CSNK1A1
Plasmid#23355DepositorInsertCSNK1A1 (CSNK1A1 Human)
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223-TYK2
Plasmid#23908DepositorInsertTYK2 (TYK2 Human)
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGM1190
Plasmid#69994PurposeOriT containing mobilizable vector for thiostrepton induced gene expression in StreptomycesDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
ExpressionBacterialAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p3xFLAG-CMV10-hUVRAG
Plasmid#24292DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUV irradiation resistance-associated gene (UVRAG Human)
Tags3xFLAGExpressionMammalianAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTagBFP2-PCNA-19-SV40NLS-4
Plasmid#55320PurposeLocalization: Nucleus, Excitation: 399, Emission: 456DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCNA-19 SV40NLS (PCNA Human)
Tags3xSV40 NLS and mTagBFP2ExpressionMammalianPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBabe-TetCMV-puro-mCherry-PA-Rac1
Plasmid#22035PurposeExpression of photoactivatable Rac1DepositorPublicationInsertmCherry-PA-Rac1 (RAC1 Avena sativa (oat), Human)
UseRetroviralExpressionMammalianMutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterTet-CMVAvailable sinceSept. 11, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy mCherry-Tubulin
Plasmid#26767DepositorPublicationAvailable sinceApril 8, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HSC1-HS4-D4Z4c-GiP
Plasmid#58541PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoterDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial
UseRetroviralExpressionMammalianPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTagBFP2-H3.3-N-14
Plasmid#55302PurposeLocalization: Nucleus/Histones, Excitation: 399, Emission: 456DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH3.3 (H3f3b Mouse)
TagsmTagBFP2ExpressionMammalianPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by