We narrowed to 15,727 results for: NOL

1. AA331

   Plasmid

   #215954

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO-GRAB_eCB2.0mut

   Plasmid

   #164607

   Purpose

   Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid non-responsive sensor GRAB_eCBmut
   

   Use

   AAV

   Mutation

   S383T, F177A

   Promoter

   hSyn

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. RS35075_pMQ30_KO_Kan

   Plasmid

   #185390

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS35075 upstream flanking homology region
   RR42_RS35075 downstream flanking homology region
   nptII kanamycin resistance marker
   

   Expression

   Bacterial

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIMVAu1

   Plasmid

   #98301

   Purpose

   Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
   

   Expression

   Yeast

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA

   Plasmid

   #205320

   Purpose

   All-in-one AAV vector expressing ISAba30 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISAba30 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-3*HA-Palmd

   Plasmid

   #216833

   Purpose

   expressing HA tag and the coding sequence of mouse Palmd

   Depositor

   Yuxuan Guo

   Article

   Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

   Insert

   Palmd (mus musculus) (Palmd Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Circular 100,50 (RAB7A)

   Plasmid

   #170117

   Purpose

   AAV vector carrying a guide RNA targeting the human RAB7A mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 100,50 guide RNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)

   Plasmid

   #139448

   Purpose

   Lentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoter

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag E2-crimson
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZDonor-Nm-Cas9-gRNA-hU6

   Plasmid

   #61366

   Purpose

   empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKII-STChRger2-TS-EYFP

    Plasmid

    #129393

    Purpose

    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    soma targeted ChRger2
    

    Use

    AAV

    Tags

    Kv2.1-TS-EYFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

    Plasmid

    #225433

    Purpose

    Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

    Depositor

    Diego Orzaez

    Article

    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

    Insert

    4xCBS:mDFR:Rep/RepA:Tnos
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Circular 200,100 with interspersed loops (SMAD4)

    Plasmid

    #180191

    Purpose

    AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAsCpf1(TATV)(BB) (pY221)

    Plasmid

    #89354

    Purpose

    Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1 RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CBh

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUSEss-CHIg-mG2b_M18

    Plasmid

    #105931

    Purpose

    Expression plasmid coding for heavy chain of mouse IgG2b antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG2b (Ighg2b Mouse)
    

    Expression

    Mammalian

    Promoter

    hEF1-HTLV prom

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_662

    Plasmid

    #216101

    Purpose

    CRISPRi, EFS-driven dCas9-KRAB (ZNF10) (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_831

    Plasmid

    #216100

    Purpose

    CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-Rabin8

    Plasmid

    #118070

    Purpose

    Expresses Flag-tagged human Rabin8 in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    human Rabin8 (RAB3IP Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-OsTIR1(WT)

    Plasmid

    #140729

    Purpose

    OsTIR1(WT)-P2A-mAID-EGFP-NES

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(WT)-P2A-mAID-EGFP-NES
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sniper-Cas9

    Plasmid

    #138559

    Purpose

    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    Sniper-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAM4941 (pCV0053)

    Plasmid

    #120083

    Purpose

    Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI
    

    Use

    Other

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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