We narrowed to 15,727 results for: NOL

1. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

   Plasmid

   #181790

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK1E-RNAP

   Plasmid

   #48144

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   rna polymerase K1E
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_eA3A-nCas9

   Plasmid

   #163535

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX Stat5B-SH2

   Plasmid

   #46516

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Stat5B (STAT5B Human)
   

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 593-692

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUSE-RapR-Src(KD)-myc

   Plasmid

   #25934

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

   Insert

   RapR-Src (Fkbp1a Mouse)
   

   Expression

   Mammalian

   Mutation

   This pUSE-RapR-Src(KD)-myc plasmid contains the k…

   Available Since

   Oct. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-ABE-NT-sgRNA

   Plasmid

   #112734

   Purpose

   AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

   Depositor

   Jin-Soo Kim

   Article

   Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

   Insert

   ABE-NT-sgRNA
   

   Use

   AAV

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

   Plasmid

   #127314

   Purpose

   Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG and PEST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Tight-Puro_Olig1

   Plasmid

   #64841

   Purpose

   A lentiviral vector for the expression of mouse Olig1 under doxycyline control

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Olig1 (Olig1 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB3052(gRNA X-4, XI-3, XII-5)

   Plasmid

   #73294

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGS1021

    Plasmid

    #59575

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    

    Use

    E. coli cloning vector

    Tags

    Twin-Strep tag

    Promoter

    None

    Available Since

    Aug. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLCHKO

    Plasmid

    #155048

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-hA3F-BE3

    Plasmid

    #113414

    Purpose

    Expresses hA3F-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3F-BE3 (APOBEC3F Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. ALC1-c007

    Plasmid

    #173112

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    CHD1L (CHD1L Human)
    

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    L613-K879, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmIL-6 mut C/EBP

    Plasmid

    #61291

    Purpose

    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site

    Depositor

    Gail Bishop

    Article

    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

    Insert

    IL-6 promoter (Il6 Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated C/EBP binding site from ACATTGTGCAATCT to…

    Promoter

    IL-6 promoter with mutant C/EBP binding site

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

15. AA025

    Plasmid

    #216005

    Purpose

    Fragmid fragment: (Cas protein) NG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-NG_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX ShcD-SH2

    Plasmid

    #46488

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcD (SHC4 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 512-623

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid

    #181788

    Purpose

    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SaCas9 TadCBEa

    Plasmid

    #193844

    Purpose

    Express TadCBEa (with SaCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    SaCas9 TadCBEa
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

    Plasmid

    #139445

    Purpose

    Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag E2-crimson
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX SH2-B-SH2

    Plasmid

    #46479

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SH2-B (SH2B1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 515-639

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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