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pGEX Grap-SH2

Plasmid

#46434

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grap (GRAP Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 51-160

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pHL 1917

Plasmid

#53024

Purpose

AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Inserts

mCherry
fimB (fimB E. coli)

Use

Synthetic Biology

Tags

Asp terminator, RBS(st7), and T1 terminator

Expression

Bacterial

Promoter

pCon and pLlacO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-Flag-Tipin

Plasmid

#23117

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pSicoR (Puro) NEMO shRNA1

Plasmid

#22505

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

NEMO shRNA1 (Ikbkg Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pGEX Cten-SH2

Plasmid

#46421

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Cten (TNS4 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 444-570

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN aa452-526

Plasmid

#73381

Purpose

Expresses amino acids 452-526 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 452-526 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa107-311

Plasmid

#73379

Purpose

Expresses amino acids 107-317 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 107-317 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pZE21-APX(W41F) dimeric

Plasmid

#58199

Purpose

Bacterial expression of APX W41F under control of TetR

Depositor

Alice Ting

Article

Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11

Insert

pea APX W41F

Expression

Bacterial

Mutation

W41F

Promoter

pL(tetO-1)

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Claspin-BPII

Plasmid

#31632

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

aa 492-622 of claspin

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
mIFP-PDHA1-N-10

Plasmid

#56236

Purpose

Localization: Mitochondrial Matrix, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

PDHA1 (PDHA1 Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
psiCheck-ApoE3'UTR

Plasmid

#41846

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

ApoE 3'UTR (APOE Human)

Use

Luciferase

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
pMX-Flag-WIP 460 deletion

Plasmid

#11773

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP aa1-460 (WIPF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

aa461-503 deleted

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
mCit-Snap25b D179K A199R

Plasmid

#53259

Purpose

SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

SNAP-25B (Snap25 Rat)

Tags

4aa-linker and mCitrine

Expression

Mammalian

Mutation

D179K and A199R

Promoter

CMV

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only
pGEX Stat2-SH2

Plasmid

#46512

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat2 (STAT2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 566-681

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX ShcC-SH2

Plasmid

#46487

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

ShcC (SHC3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 485-594

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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mPapaya1-Zyxin-6

Plasmid

#56655

Purpose

Localization: Focal Adhesions, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Zyxin (ZYX Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya1-CENPB-N-22

Plasmid

#56351

Purpose

Localization: Nucleus/Centromeres, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

CENPB (CENPB Human)

Tags

mPapaya1

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya1-Vimentin-7

Plasmid

#56654

Purpose

Localization: Intermediate Filaments, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Vimentin (VIM Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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3604 pGEX4T-1 Bad S136A (104-141)

Plasmid

#8801

Depositor

Stanley Korsmeyer

Article

Harada et al Mol Cell 1999 Apr;3(4):413-22.

Insert

Bad (Bad Mouse)

Tags

GST

Expression

Bacterial

Mutation

Contains AA's 104-141; S136A

Promoter

Ptac

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
Mouse c-myc (exon I)

Plasmid

#8446

Depositor

Frederic Mushinski

Article

Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

Insert

Mouse c-myc (exon I) (Myc Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only