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pKB01_mCherry

Plasmid

#69104

Purpose

Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Blast

Plasmid

#227270

Purpose

Empty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xHA-Reptin

Plasmid

#51636

Purpose

Mamamlian expression of 3xHA tagged reptin, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

reptin (RUVBL2 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-LgBiT-SpyCatcher

Plasmid

#223634

Purpose

Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His6, LgBiT

Expression

Bacterial

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pHS2002

Plasmid

#239832

Purpose

Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

NovaIscB omegaRNA scaffold

Expression

Mammalian

Mutation

Replacement of A12-A57 with GAAA in omegaRNA scaf…

Promoter

human U6

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pALOD4 non-binding mutant

Plasmid

#111027

Purpose

Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

Depositor

Arun Radhakrishnan

Article

Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

Insert

Domain 4 of Anthrolysin O

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: S404C, C472A, G501A, T502A, T503A, L5…

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuclear-EKAR4

Plasmid

#174439

Purpose

Nuclear-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

nuclear-EKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDendra2-H2b

Plasmid

#157817

Purpose

Fluorescent reporter for histone H2B tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

Histone H2B (H2bc3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt1(WT)

Plasmid

#86637

Purpose

Expresses eGFP-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEF1a-Ronin-FLAG-IRES-Neo

Plasmid

#28020

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Insert

Ronin (THAP11 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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CD8a

Plasmid

#86050

Purpose

A mammalian expression plasmid encoding CD8a without tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Expression

Mammalian

Promoter

CMV, T7

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDendra2-H4

Plasmid

#157815

Purpose

Fluorescent reporter for histone H4 tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H4 (H4c14 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDendra2-H3

Plasmid

#157814

Purpose

Fluorescent reporter for histone H3 tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H3 (H3c1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TCF1

Plasmid

#40620

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

TCF7 (TCF7 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pHsp18.2 (GB0153)

Plasmid

#68171

Purpose

Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp18.2 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP670-HRasCT

Plasmid

#176030

Purpose

Encoding membrane-targeted miRFP670

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

Membrane-targeted miRFP670

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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CAGGS-MCS-NLS-3XFLAG-V5

Plasmid

#196383

Purpose

Empty backbone vector to insert cDNA for strong expression in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Type

Empty backbone

Tags

3xFLAG and V5

Expression

Bacterial and Mammalian

Promoter

CAGGs

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-PH1(WT)

Plasmid

#86599

Purpose

Expresses eGFP-tagged PH(WT) domain of human Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 PH domain (AKT1 Human)

Expression

Mammalian

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-GalT

Plasmid

#11929

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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FRB-GFP-GCN4-ppKin14

Plasmid

#240427

Purpose

-end oriented kinesin for rapalog induced pulling experiments.

Depositor

Anna Akhmanova

Article

Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

Insert

ppKin14Vib

Tags

FRB, GCN4, and GFP

Expression

Mammalian

Mutation

deleted amino acids 1-850

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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