We narrowed to 15,730 results for: NOL

1. pGS1022

   Plasmid

   #59576

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
   

   Use

   E. coli cloning vector

   Tags

   Twin-Strep tag

   Promoter

   None

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. BCL6-H2Kk-RV (H2K-BCL6)

   Plasmid

   #40352

   Depositor

   Alexander Dent

   Article

   Mondal et al J Immunol. 2010 Apr 15;184(8):4123-32. E

   Insert

   BCL6 (BCL6 Human)
   

   Use

   Retroviral

   Tags

   IRES mouse H2Kk

   Expression

   Mammalian

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCfB3039(XII-2 MarkerFree)

   Plasmid

   #73279

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUPD2 HDV (GB1444)

   Plasmid

   #160576

   Purpose

   Hepatitis Delta Virus ribozyme signal for the assembly of gRNA expression cassettes.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   HDV
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p1.2-Zeo-eGFP

   Plasmid

   #162743

   Purpose

   Fluorescent reporter for CHO cells expression studies

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Insert

   enhanced green fluorescent protein
   

   Expression

   Mammalian

   Mutation

   consensus Kozak sequence (GCCGCCATGG) added befor…

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX Nck-SH2

   Plasmid

   #46457

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Nck (NCK1 Human)
   

   Tags

   GST and Thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acids 275-377

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 PA-mCit

   Plasmid

   #113443

   Purpose

   ProteinA-mCitrine control expression vector

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   ProteinA-mCitrine
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGMC00013

   Plasmid

   #172525

   Purpose

   sgRNA against mouse Mr1

   Depositor

   Raj Chari

   Article

   Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

   Insert

   Mr1 (Mr1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ269A2

   Plasmid

   #164714

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI
   

   Use

   CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX ShcA-SH2

    Plasmid

    #46486

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcA (SHC1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 476-584

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ265

    Plasmid

    #164712

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    rAPOBEC1-zCas9(D10A)-UGI
    

    Use

    CRISPR; Gateway compatible rapobec1-cbe_v01 entry…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1352wUV2 F3bbs kan NNNTGGGCGk

    Plasmid

    #18756

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-dCpf1-BE0

    Plasmid

    #107678

    Purpose

    Expresses dLbCpf1-BE0 in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE0
    

    Expression

    Mammalian

    Mutation

    D832A/E925/D1148A

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCellFree_G03 FOXM

    Plasmid

    #67118

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FOXM (FOXM1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pNHT7::RPL15::sNluc2

    Plasmid

    #186763

    Purpose

    An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPSP6+t-gfp

    Plasmid

    #48130

    Purpose

    Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    gfp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCellFree_G03 GTF2E1

    Plasmid

    #67089

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2E1 (GTF2E1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. 1352wUV2 F1bbs kan GCGTGGNNN

    Plasmid

    #18753

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCellFree_G03 EBNA1BP2

    Plasmid

    #67072

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    EBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Intersectin I SH3 B domain (human)

    Plasmid

    #47414

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain B (ITSN1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain B

    Promoter

    tac

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only