165,921 results

1. pDD131

   Plasmid

   #119886

   Purpose

   Optimized aTc inducible pTet promoter for Rhodococcus opacus expressing mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

   Insert

   pTet_mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Emerin pEGFP-C1 (637)

   Plasmid

   #61993

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Emerin (EMD Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAK_EF1a_CasRx_Hygro

   Plasmid

   #134840

   Purpose

   Lentiviral expression of CasRx T2A Hygromycin

   Depositor

   Michael Yaffe

   Insert

   RfxCas13d
   

   Use

   CRISPR and Lentiviral

   Tags

   T2A Hygromycin

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Sport6-CD9-pHluorin

   Plasmid

   #130905

   Purpose

   Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD9-pHluorin (CD9 Aequorea victoria, Human)
   

   Use

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   phluorin inserted between Asn-50 and Asn-51 in ex…

   Promoter

   CMV

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TRE-DIO-eYFP (AAV1)

   Viral Prep

   #117383-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-TRE-DIO-eYFP (#117383). In addition to the viral particles, you will also receive purified pAAV-TRE-DIO-eYFP plasmid DNA. Tet-inducible, Cre-dependent eYFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Promoter

   TRE

   Tags

   eYFP (Tet-inducible, Cre-dependent)

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-PM-TORCAR

   Plasmid

   #64930

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   Lyn-TORCAR (EIF4EBP1 Human, Synthetic)
   

   Use

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE

   Plasmid

   #89609

   Purpose

   over-expression of VEGF-A(165) in human cells

   Depositor

   Fernando Fierro

   Insert

   VEGF-A(165) (VEGFA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   K188E

   Promoter

   MNDU3

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDsRed1-CMV-Rat Arc-DsRed1-pA

   Plasmid

   #233049

   Purpose

   To Express a Rat Arc-DsRed fusion protein from a CMV promoter

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Neurobiol Learn Mem. 2015 Oct;124:34-47.

   Insert

   rat Arc-DsRed fusion (Arc Rat)
   

   Use

   Tags

   DsRed1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. vGlut1 peptide- GST

   Plasmid

   #218677

   Purpose

   Expresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesicles

   Depositor

   Axel Brunger

   Article

   Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

   Insert

   vGlut (Slc17a6 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162381

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    CAG

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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