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We narrowed to 14,321 results for: cas9

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Showing: 841 - 860 of 14321 results

  6. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    135014-plasmid-map-sequence-id-267599

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  12. PB-iCas9

    Plasmid
    #160048
    Purpose
    inducible expression of Cas9
    Depositor
    Xiaojun Lian
    Insert
    Cas9
    Expression
    Mammalian
    Promoter
    TRE
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    160048-plasmid-map-sequence-id-313847

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  15. pMZ376

    Plasmid
    #74213
    Purpose
    Combined sgRNA/Cas9 vector with KanMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74213-plasmid-map-sequence-id-139302

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  16. FUG-T2A-Cas9

    Plasmid
    #75346
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    75346-plasmid-map-sequence-id-142581

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  17. Cas9 ROSA sgRNA mCherry

    Plasmid
    #155280
    Purpose
    Lentiviral Cas9 expression, ROSA sgRNA expression, mCherry marker
    Depositor
    Martin Carroll
    Insert
    spCas9
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155280-plasmid-map-sequence-id-309987

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  18. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Publication
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    138147-plasmid-map-sequence-id-349848

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  19. M-tdTom-SP

    Plasmid
    #48677
    Purpose
    Mammalian tdTomato activation reporter for SP with GTCCCCTCCACCCCACAGTG protospacer
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Insert
    TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9
    Use
    CRISPR
    Promoter
    Sp6
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  20. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid
    #139086
    Purpose
    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    139086-plasmid-map-sequence-id-273882

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Showing: 841 - 860 of 14321 results