We narrowed to 166,303 results for: addgene

1. MSP2133

   Plasmid

   #72249

   Purpose

   Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   Y450A, N497A, R661A, Q695A, and Q926A

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-CAG-EGFP barcode-0-SV40 polyA

   Plasmid

   #190864

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode0
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-EFS-DIO-GRAB_ATP1.0

   Plasmid

   #167581

   Purpose

   Express the ATP sensor GRAB_ATP1.0 in Cre positive cells

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP sensor GRAB_ATP1.0 (P2RY1 Human)
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEM-Cas9HF1-indRecA56

   Plasmid

   #102294

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CAG-EGFP barcode-3-SV40 polyA

   Plasmid

   #190867

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode3
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CAG-EGFP barcode-4-SV40 polyA

   Plasmid

   #190868

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode4
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. qTAG-C-miRFP670nano3-Blast-H2BC11

   Plasmid

   #207762

   Purpose

   Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. U6GRNA

   Plasmid

   #68370

   Purpose

   gRNA scaffold with hU6 promoter

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PX458_KMT2B_2

   Plasmid

   #101075

   Purpose

   Encodes gRNA for 3' target of human KMT2B

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against KMT2B (KMT2B Human)
   

   Use

   CRISPR

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PX458_KMT2B_1

    Plasmid

    #101074

    Purpose

    Encodes gRNA for 3' target of human KMT2B

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KMT2B (KMT2B Human)
    

    Use

    CRISPR

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pB PUb-integrase

    Plasmid

    #183966

    Purpose

    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    PhiC31 integrase
    

    Use

    Piggybac insect transgenesis destination vector

    Promoter

    Aedes aegypti Polyubiquitin

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CAG-EGFP barcode-2-SV40 polyA

    Plasmid

    #190866

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode2
    

    Use

    AAV

    Mutation

    WT

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pICSL11056

    Plasmid

    #68258

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

    Plasmid

    #226011

    Purpose

    CasRx guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. phROSA26-Tet-HA-JLP_WT

    Plasmid

    #208770

    Purpose

    Tetracycline inducible expression vector for HA-tagged wild-type JLP protein (HA-JLP_WT), used as a donor plasmid for HA-JLP_WT knock-in at the human ROSA26 locus

    Depositor

    Katsuji Yoshioka

    Article

    Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

    Insert

    JLP (SPAG9 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP gRNA (BRDN0000562805)

    Plasmid

    #80035

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. plenti-UBC-HA-TMEM230

    Plasmid

    #225516

    Purpose

    Lentiviral TMEM230 expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. plenti-UBC-HA-TMEM230triplemut

    Plasmid

    #225521

    Purpose

    Lentiviral TMEM230triplemut expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Mutation

    Y92C/R141L/X184W

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDH-EF1-FHC-POLQ-DM

    Plasmid

    #64880

    Purpose

    lentiviral expression of human POLQ double mutant (in POL and HDL domains)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M and D2330A,Y2331A

    Promoter

    EF1alpha

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSP2135

    Plasmid

    #72248

    Purpose

    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, and D1135E

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge