We narrowed to 166,303 results for: addgene

1. PTPRA-MAC

   Plasmid

   #139632

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   PTPRA (PTPRA Human)
   

   Tags

   StrepIII-HA-BirA*

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

   Plasmid

   #168107

   Purpose

   PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Insert

   Cas9-P2A-mCherry
   

   Use

   CRISPR and Cre/Lox

   Tags

   HA

   Promoter

   CAG

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

   Plasmid

   #225947

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis, Evan Reid

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S3E S17E S19E

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

   Plasmid

   #225948

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis, Evan Reid

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S3A S17A S19A

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-Puro-TNNT2-Neo

   Plasmid

   #214013

   Purpose

   Expression of cardiac troponin T

   Depositor

   Paul Burridge

   Article

   Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

   Insert

   TNNT2 (TNNT2 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS2155

   Plasmid

   #141065

   Purpose

   AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

   Insert

   SYN1-EmGFP-WPRE
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   April 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. p4A8_S7T_BC

   Plasmid

   #217832

   Purpose

   4A8 variant in barcoded, yeast surface display plasmid

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Insert

   4A8 variant Fab
   

   Expression

   Yeast

   Mutation

   VL: S7T

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pyc111m

   Plasmid

   #158779

   Purpose

   ribosome-tagged, Cre-dependent GCaMP for soma localization

   Depositor

   Jennifer Garrison, Zachary Knight

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   GCaMP6m-RPL10a
   

   Use

   AAV

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-sh-mSOX9-2

   Plasmid

   #40645

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   sh-mSOX9-2 (Sox9 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pBabe WT Akt2

    Plasmid

    #14551

    Depositor

    Joan Brugge

    Article

    Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

    Insert

    Akt2 (AKT2 Human)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

11. LLP109 pEF1a-gRNA-GFP

    Plasmid

    #100935

    Purpose

    GFP, puromycin and gRNA scaffold driven by EF1a promoter

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA scaffold
    

    Use

    Grna scaffold

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PEA1

    Plasmid

    #176527

    Purpose

    Delivers all prime editing nickase components in a single plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CbH for Cas9, hU6 for gRNAs

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP788_Traptavidin-EZH2-FL-CO

    Plasmid

    #211781

    Purpose

    AviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-EZH2
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. OVA-miSFIT-17-T

    Plasmid

    #124677

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV PIG C/EBPa

    Plasmid

    #66985

    Purpose

    Retroviral expression plasmid for mouse C/EBPa expression; containing bicistronic IRES-GFP

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    C/EBP alpha (Cebpa Mouse)
    

    Use

    Retroviral

    Promoter

    LTR

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-human NMyc-humanER-IRES-mCherry

    Plasmid

    #73571

    Purpose

    Mammalian retroviral expression of NMyc-ER

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    NMyc (MYCN Human)
    

    Use

    Retroviral

    Tags

    Human ER and IRES-mCherry

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pMXs-ms-Sox18

    Plasmid

    #50782

    Purpose

    retroviral expression of mouse Sox18

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Sox18 (Sox18 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-cGAS C396/7A-HA

    Plasmid

    #130917

    Purpose

    Expresses human cGAS-HA with C396/397A point mutations; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGAS C396/7A
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    mutated residues 396 and 397 to alanines

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Fyn-Fyn Biosensor

    Plasmid

    #124655

    Purpose

    FRET biosensor. Fyn-tagged biosensor targeting to membrane

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

    Insert

    Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-sh-mSlug-4

    Plasmid

    #40648

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSlug-4 (Snai2 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only