We narrowed to 33,204 results for: multi
-
Plasmid#99884PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
DS-ST1cas
Plasmid#48647PurposeBacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
ST1 tracrRNA
UseCRISPRPromoterproCAvailable sinceNov. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
DHFR-PP7-VP64_T2A_GFP
Plasmid#86167PurposeExpresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cellsDepositorInsertDHFR-PP7-VP64_T2A_GFP
UseCRISPR and LentiviralTagsDestabilized domain (N-terminus version) of E.col…ExpressionMammalianPromoterEF1aAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEntr-SRFflbio
Plasmid#32971DepositorAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSET-dCas9-actII-4-NT-S1
Plasmid#110185PurposeRepression of gene expression in Streptomyces by CRISPRiDepositorInsertsdCas9
sgRNA
UseCRISPR; IExpressionBacterialMutationD10A, H840APromoterJ23119 and ermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRDA_887
Plasmid#201164PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD157
Plasmid#84033PurposeVector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafishDepositorInsertsCre
Venus
UseCre/Lox; Zebrafish transgenesisTagsmCherryPromoterCrystalin and fabp10Available sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Cytbow
Plasmid#158995PurposeMulticolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)DepositorInsertCytbow
TagsThe default expressed EBFP2 is fused to H2BExpressionMammalianPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MS2-Snapf
Plasmid#65860PurposeExpresses RNA-binding protein MS2 with fast SNAP fusion tagDepositorInsertsMS2
Fast SNAP
TagsHA and NLSExpressionBacterial and YeastAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEQ111m
Plasmid#40190DepositorInsertEcoRI endonuclease & EcoRI methylase
ExpressionBacterialMutationchanged Glutamic Acid 111 to GlutaminePromoterLambda PLAvailable sinceNov. 19, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-DIO-SYP-HRP
Plasmid#117186PurposePlasmid name in publication: pAAV-DIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertSYP-HRP
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-flag-GFP-iCAM-1
Plasmid#205186PurposeThis vector encodes of a synCAM (iCAM1) and GFPDepositorInsertsynCAM iCAM1 and GFP
UseLentiviralExpressionMammalianAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-mTagBFP2
Plasmid#216128PurposeLentiviral vector for expressing mTagBFP2 localizing to the nucleusDepositorInsertEF1a-NuclearLocalizationSignal-mTagBFP2-WPRE
UseLentiviralExpressionMammalianAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP
Plasmid#102797PurposeRetrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroRDepositorInsertssgRNA
EGFP
UseCRISPR and RetroviralExpressionMammalianMutationH231LAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE:iRFP670-EFS:Cre-2A-GFP
Plasmid#68477PurposeInducible lentivirus expressing iRFP670, Cre, and GFPDepositorInsertsiRFP670
Cre-2A-GFP
UseLentiviralExpressionMammalianPromoterEFS and pTREAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
iChr2-Control-Mosaic (SO274)
Plasmid#99751PurposeSecond generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteinsDepositorInsertHIs-H2B-Cherry, H2B-EGFP, HA-H2B-Cerulean
UseCre/Lox and Mouse TargetingExpressionMammalianAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGMCK-T38S38-750-luxAB-DAS+4
Plasmid#49518PurposeKanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 siteDepositorPublicationInsertT38S8-P750-luxAB-DAS+4
TagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialPromoterP750Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47454PurposeepiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.DepositorInsertCRY2PHR-NLS-SID4X
UseAAVTags2A_phiLOV2.1ExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS413-GAL1-yEGFP1
Plasmid#40235DepositorInsertyEGFP1
TagsHIS3MX6ExpressionBacterialPromoterGAL1Available sinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEVOL_PylRS(WT)-tRNA
Plasmid#223512PurposePylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codonDepositorInsertsTags6xHisExpressionBacterialPromoteraraBAD, rrnB, and glnS and proKAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only