We narrowed to 11,829 results for: 158
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sfGFP-SasG(8G)-mCherry
Plasmid#206950PurposeMammalian expression of heterobifunctional SasG linkers (8G)DepositorInsertsfGFP-SaSasG (8G)-mCherry
TagsmCherry and sfGFPExpressionMammalianAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
K401-Micro
Plasmid#122485PurposeComponent of optically-induced motor dimerization systemDepositorInsertHis tag MBP Kinesin 1-401 SspB micro
TagsH6-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-401Available SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL437 AAVS1 EQR sgRNA
Plasmid#126447PurposeTo cut the human AAVS1 locus for integration.DepositorInsertspacer against human AAVS1 safe-harbor locus with NGAG PAM
ExpressionMammalianPromoterhuman U6Available SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Tbxas1
Plasmid#170305PurposeA knock-out vector for the mouse Tbxas1DepositorInsertA gRNA targeting the mouse Tbxas1 gene.
UseCRISPR and LentiviralAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
P9_TEVs_cLuc
Plasmid#119303PurposeExpresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logicDepositorInsertsplit cLuc connected to P9 coil via TEVs cleavable linker
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
NO- DSD Sensor 3
Plasmid#199787PurposeSynthetic gene circuitDepositorInsertNO- DSD Sensor
UseSynthetic BiologyAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)
Plasmid#181787PurposeExpresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShTnsB, ShTnsC, ShCas12k-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKH162_FLAG-APEX2-YIPF3 LIR mut
Plasmid#214981PurposeFlag-APEX2 tagged LIR mutant YIPF3DepositorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDule-IBBN (G2)
Plasmid#85500PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR284
Plasmid#125093PurposeExpression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143DepositorInsertsompR(REC)-manR(DBD)122
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgMYC guide 2
Plasmid#193597PurposecMYC knockoutDepositorInsertsgMYC guide 2 (MYC Human)
UseLentiviralAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA, EGFP Spastin M87 with exon 4, N386C
Plasmid#167543PurposeFor doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4, with N386C mutation.DepositorInsertSpastin M87 with exon 4 (SPAST Human)
TagsEGFP and HA tagExpressionMammalianMutationN386CPromoterCMVAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPLINTR BFP v1 backbone
Plasmid#179779PurposeSPLINTR backbone vectorDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-ER-EmEGFP
Plasmid#169000PurposeExpress ER-EmEGFP in zebrafishDepositorInsertER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)
UseUnspecifiedTagsEGFPAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
VQR variant
Plasmid#138560PurposeExpresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSDDepositorInsertVQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, R1335Q, T1337RPromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-H2B-6
Plasmid#54111PurposeLocalization: Nucleus/Histones, Excitation: 487, Emission: 509DepositorInsertH2B (H2BC11 Human)
TagsmEmeraldExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable SinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSR241
Plasmid#125091PurposeExpression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181DepositorInsertsompR(REC)-yxdJ(DBD)137
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0373 (His-MBP-TniQ)
Plasmid#130638PurposeExpresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.DepositorInsertVchTniQ
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on TniQExpressionBacterialPromoterT7Available SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-evoA1-seBEN_empty
Plasmid#174701PurposeSmall-molecule controlled base editingDepositorInsertLenti-evoA1-seBEN
UseLentiviralTagsmyc tagPromoterEFS/U6Available SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only
