We narrowed to 13,357 results for: BASE
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Plasmid#173017PurposeImproved green-red FRET based cAMP indicator.DepositorInsertGR-ICUE2
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3p
Plasmid#162457PurposePositive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.DepositorInsertAga2p-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsALFA tag, HA and myc tags, and eUnaG2 fluorescent…PromoterGAL1Available SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3-NLS
Plasmid#138218PurposeNuclear-targeted FRET-based cAMP indicator.DepositorInsertICUE3-NLS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272
Plasmid#154829PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietinDepositorInsertscoEPO
coEPO
UseCre/LoxExpressionMammalianMutationCodon-optimized (Cricetulus griseus)Promoter100RPU.2Available SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Golgi-DKAR
Plasmid#49790PurposeFRET-based, Golgi-targeted kinase activity reporter for PKDDepositorInsertD kinase activity reporter
TagsCFP, YFP, and eNOS(1-33)ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V
Plasmid#119723PurposeAAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorInsertsCaMPARI2
CaMPARI2
UseAAVTagsNES-hisExpressionMammalianMutationF391W, L398VPromoterhSyn1Available SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNubWtXgate
Plasmid#105079PurposeYeast mating-based Split Ubiquitin Assay, positive controlDepositorTypeEmpty backboneTagsNubIExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
F40 tension sensor
Plasmid#101252PurposeExpresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.DepositorInsertYPet(short)-F40-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianPromoterCMVAvailable SinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RasAR-NES
Plasmid#205567PurposeGenetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.DepositorInsertRasAR-NES (RAF1 Synthetic, Human)
TagsNuclear export signal (NES)ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394
Plasmid#128387PurposeExpresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruptionDepositorUseLentiviral, Luciferase, and Synthetic BiologyTagsCLuc394 and NLuc398ExpressionMammalianPromoterEFS and IRESAvailable SinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-evoCjCas9-ABE8e-BlastR
Plasmid#194060PurposeMammalian expression of evoCjCas9 ABE8eDepositorInsertevoCjCas9-ABE8e
UseCRISPRExpressionMammalianAvailable SinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD68Kan2-AphA
Plasmid#191248PurposeRSF1010-based broad host-range plasmid engineered from pAM5409, kanamycin resistanceDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-GMR-eya(shRNA)
Plasmid#157991PurposepBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.DepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
SL016: AAV-CAG-FLEX-CheRiff-GFP
Plasmid#124776PurposeFor AAV based expression of CheRiff in Cre-Positive cellsDepositorInsertCheRiff-GFP
UseAAV and Cre/LoxTagsGFPPromoterCAGAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)
Plasmid#79619PurposeExpresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
Tags3xFlag-PmCDA1ExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailable SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2225
Plasmid#67553PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2337
Plasmid#67555PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-K-red-SPOTIT
Plasmid#191490PurposeRed fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoterDepositorInsertK-red-SPOTIT
UseAAVPromoterCAGAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL6
Plasmid#48746PurposeExpression of luciferase driven by mPer2 promoter region (bases -1128 to +2129 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region (-1128 to +2129)
UseLuciferaseAvailable SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
MZB223
Plasmid#228491PurposeEmpty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneTags8xHisExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only