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Plasmid#237663PurposeFor integration of EGFP-NPM1-KS-FtoGDepositorInsertNPM1-KS-FtoG (NPM1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
ADEPT-pTarget-tetR
Plasmid#238043PurposeModified ADEPT-pCas9 with sfGFP under TtrB promoter for tetrathionate (TTR) sensing.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterttrBAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJAK184.tetR.PT5-3.dCas9
Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRTagsExpressionBacterialMutationPromoterTetR-PT5-3Available sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-MCS
Plasmid#210527Purposedoxycycline-inducible expression of Human inserts (MCS) in mammalian cellsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-no-Flag-TET1-S-WT
Plasmid#232941PurposeExpresses short isoform of TET1 in mammalian cells in its wild-type formDepositorInsertTET1-S-WT (TET1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-AncSoxCEF
Plasmid#231526PurposeExpresses reconstructed Ancestral SoxCEF in mammalian cells, for lentivirus generationDepositorInsertAncestral SoxCEF HMG flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-AncSoxB
Plasmid#231524PurposeExpresses reconstructed Ancestral SoxB in mammalian cells, for lentivirus generationDepositorInsertAncestral SoxB HMG flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-AncSoxBCEF
Plasmid#231525PurposeExpresses reconstructed Ancestral SoxBCEF in mammalian cells, for lentivirus generationDepositorInsertAncestral SoxBCEF HMG flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
rTetR-GFP-p65
Plasmid#232633Purposep65 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline additionDepositorInsertrTetR-GFP-p65 (RELA Human, Synthetic)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_mScarlet_Convergent
Plasmid#229790PurposeBxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_mScarlet_Divergent
Plasmid#229791PurposeBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
psBIG1a ActBT2ATetP2AP - mAGH
Plasmid#229973PurposeHarbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-HygroDepositorInsertActBT2ATetP2AP - mAGH
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTripZ-Tet-ON mCherry
Plasmid#187262PurposeDoxycyclin inducible expression of mCherryDepositorInsertmCherry-NLS
UseLentiviralTagsmCherryExpressionMutationPromoterCMVAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-3xFlag-dCas13-mTET2HxDCD
Plasmid#228235PurposeFor targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13DepositorInsertsUseCRISPRTagsExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterCAGAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA
Plasmid#225350Purposepiggyback backbone for non-fluorescent GFP tether and H2B-IRFP expressionDepositorInsertH2B (H2B Human, Synthetic)
UseTagsmiRFP and non-fluorescent GFP (Y67F)-P2AExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-Neo-TetO-Cdk13_Untagged
Plasmid#127344PurposeUntagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vectorDepositorInsertCyclin Dependent Kinase 13 (Cdk13 Mouse)
UsePiggybac transposase vectorTagsNoneExpressionMammalianMutationBase pairs 1-1554 of transgene are codon optimize…PromoterTetO PromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-Neo-TetO-Cdk13_Nterm_FlagHa
Plasmid#127345PurposeN-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vectorDepositorInsertCyclin Dependent Kinase 13 (Cdk13 Mouse)
UsePiggybac transposase vectorTagsFlag- HA- Tandem EpitopesExpressionMammalianMutationBase pairs 1-1554 of transgene are codon optimize…PromoterTetO PromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL2620 MTK3-TetR
Plasmid#226670PurposeMTK3 part containing the coding sequence of TetRDepositorInsertTetR
UseSynthetic BiologyTagsExpressionMutationPromoternoneAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-human cGASdeltaN-HA
Plasmid#186897PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Human, Synthetic)
UseRetroviralTagsHAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-orangutan cGASdeltaN-HA
Plasmid#186898PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertOrangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Pongo abelii, Synthetic)
UseRetroviralTagsHAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only