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pBB542

Plasmid

#27395

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

dnaK, dnaJ, groESL

Expression

Bacterial

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pECJ3

Plasmid

#75465

Purpose

Toggle switch plasmid containing lacI-pdt3

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

lacI-pdt3

Expression

Bacterial

Promoter

pLtetO

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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GPI-mEGFP

Plasmid

#182866

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

GPI-mEGFP

Expression

Mammalian

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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mAzurite-N1

Plasmid

#54617

Purpose

Localization: N1 Cloning Vector, Excitation: 384, Emission: 450

Depositor

Patrick Daugherty, Michael Davidson

Article

Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

Type

Empty backbone

Tags

mAzurite

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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Opie2-PuroR

Plasmid

#131613

Purpose

Expresses PuroR and dsRed.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR
dsRed

Expression

Insect

Promoter

Opie2 and hr5Ie1

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBB550

Plasmid

#27396

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

dnaK, dnaJ, GroESL

Expression

Bacterial

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pH2B_mCherry_IRES_neo3

Plasmid

#21044

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-pvc10

Plasmid

#243750

Purpose

For affinity purification of spike tip (Pvc10)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEZYflag

Plasmid

#18700

Purpose

pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pZE27GFP3

Plasmid

#75456

Purpose

Plasmid for constitutive expression of gfp-pdt3

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt#3

Expression

Bacterial

Promoter

placIq promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2

Plasmid

#79783

Purpose

Expresses Spinach2 under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEPkan-S

Plasmid

#41017

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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mAzurite-C1

Plasmid

#54583

Purpose

Localization: C1 Cloning Vector, Excitation: 384, Emission: 450

Depositor

Patrick Daugherty, Michael Davidson

Article

Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

Type

Empty backbone

Tags

mAzurite

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET31b-T7-Spinach2-Ct

Plasmid

#79158

Purpose

Expresses the Ct biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Ct

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Dp

Plasmid

#79159

Purpose

Expresses the Dp biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Dp

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMyrPalm_mEGFP

Plasmid

#21037

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pMyrPalm

Tags

mEPFP

Expression

Bacterial and Mammalian

Available Since

May 12, 2009

Availability

Academic Institutions and Nonprofits only

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pKmK.C1

Plasmid

#125059

Purpose

Storage plasmid for K.marxianus centromere

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

KmARS7/CenD

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.C2

Plasmid

#125060

Purpose

Storage plasmid for K.marxianus centromere

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

KmARS/CEN5

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.C3

Plasmid

#125061

Purpose

Storage plasmid for K.marxianus centromere

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

KmARS/CEN6

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1029

Plasmid

#22066

Depositor

Emmanuelle Bouveret

Article

Battesti et al Proteomics. 2008 Nov . 8(22):4768-71.

Type

Empty backbone

Tags

T25-Flag

Expression

Bacterial

Available Since

Oct. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pKmK.C4

Plasmid

#125062

Purpose

Storage plasmid for K.marxianus ARS

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

KmARS2

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-Purg1-3FLAG

Plasmid

#19356

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

urg1 promoter

Expression

Yeast

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pBADZ-HisCre

Plasmid

#111187

Purpose

Helper plasmid containing Cre-recombinase gene, which is expressed in DH10MultiBac cells

Depositor

Tim Richmond

Article

Berger et al Nat Biotechnol. 2004 Dec;22(12):1583-7.

Insert

Cre recombinase

Tags

His tag

Expression

Bacterial

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-KanMX6-Purg1-3FLAG

Plasmid

#19354

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

urg1 promoter

Expression

Yeast

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pEB1030

Plasmid

#22067

Depositor

Emmanuelle Bouveret

Article

Battesti et al Proteomics. 2008 Nov . 8(22):4768-71.

Type

Empty backbone

Tags

T18-Flag

Expression

Bacterial

Available Since

Oct. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512g3.1

Plasmid

#171200

Purpose

Expresses Br512g3.1 polymerase in E.coli

Depositor

Andrew Ellington

Article

Bhadra et al medRxiv 2022.08.09.22278605

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (vHP47…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pH3U3-mcs

Plasmid

#12609

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Multiple cloning site in His3 Ura3 reporter plasmid

Expression

Bacterial

Available Since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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pEZYegfp

Plasmid

#18671

Purpose

pEGFPC4 converted Gateway Destination vector for expressing EGFP tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pMH0004

Plasmid

#174087

Purpose

UCOE-SFFV-Cas9-BFP

Depositor

Jonathan Weissman

Article

Hein et al Nat Biotechnol. 2022 Mar;40(3):391-401.

Insert

Cas9-BFP

Use

Lentiviral

Promoter

SFFV

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pZE27MC3

Plasmid

#75464

Purpose

Plasmid for constitutive expression of mcherry-pdt3

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mcherry-pdt#3

Expression

Bacterial

Promoter

placIq promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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