163,546 results

1. pLminP_Luc2P_RE1

   Plasmid

   #90335

   Purpose

   NFkB - p50/p65 pathway gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   NFKB

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRIPZ-bioGEF-UBwt-PURO

   Plasmid

   #208045

   Purpose

   Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   Ub (UBC Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Expression

   Mutation

   Promoter

   tetO/UBC

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_FBXW7_WT

   Plasmid

   #81795

   Purpose

   Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   FBXW7 (FBXW7 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV8)

   Viral Prep

   #104492-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Tags

   None

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

5. pCXLE-EGFP

   Plasmid

   #27082

   Purpose

   Integration-free (episomal) expression of EGFP

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFCIV-CEACAM6

   Plasmid

   #149303

   Purpose

   lentiviral packaging vector for CEACAM6 expression.

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

   Insert

   CEACAM6 (CEACAM6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ubiquitin C

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-hM4D(Gi)-mCherry (AAV8)

   Viral Prep

   #50475-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCherry

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

8. TagBFP_A1aY1

   Plasmid

   #158752

   Purpose

   Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

   Insert

   TagBFP-T_A1aY1
   

   Use

   Lentiviral

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG (CMV enhancer and chicken beta actin promoter)

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b-AavLEA1

   Plasmid

   #53093

   Purpose

   AavLEA1 expression (in BL21 DE3 E. coli strain)

   Depositor

   Claude Férec

   Article

   Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

   Insert

   AavLEA1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGF-GAS4X

    Plasmid

    #225751

    Purpose

    This plasmid was designed to selectively monitor the activation of the STAT1 signaling pathway in NK cells, a pathway known to play a crucial role in regulating NK cells' activation.

    Depositor

    Eun Hee Han

    Article

    Min et al Commun Biol. 2024 Sep 30;7(1):1222. doi:

    Insert

    interferon gamma activating sequence (GAS) (STAT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-FLAG-REL

    Plasmid

    #27253

    Depositor

    Thomas Gilmore

    Article

    Leeman et al Oncogene. 2008 Dec 4. 27(53):6770-81.

    Insert

    REL (REL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

    Plasmid

    #234952

    Purpose

    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. piRFP-N3-Tyrosinase

    Plasmid

    #80152

    Purpose

    Melanosome marker

    Depositor

    Santiago Di Pietro

    Article

    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

    Insert

    Tyrosinase (TYR Human)
    

    Use

    Tags

    iRFP682

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pmEGFP_a_tubulin_C1

    Plasmid

    #21039

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    pmEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. scAAV-Cre

    Plasmid

    #177933

    Purpose

    Expresses Cre-recombinase and can be packaged into AAV particles.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Insert

    CAG-Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5/FRT/TO_H2B_HaloTag7

    Plasmid

    #169329

    Purpose

    Expression of H2B-HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag7 (H2BC21 Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSFFV-SpyTag-sfCherry2(11)-TagBFP

    Plasmid

    #117485

    Purpose

    Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    SpyTag002-GG-sfCherry2(11)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV1)

    Viral Prep

    #162377-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Tags

    None

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pRDA_478

    Plasmid

    #179096

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (SpG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Syn-ChrimsonR-tdT (AAV9)

    Viral Prep

    #59171-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    Syn

    Tags

    tdTomato

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

21. LPC-flag-REST-WT

    Plasmid

    #41903

    Depositor

    Stephen Elledge

    Article

    Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

    Insert

    RE1-silencing transcription factor (REST Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 6xHis-MBP-BrCas12b-RFND

    Plasmid

    #195339

    Purpose

    Expresses Engineered BrCas12b in Bacteria

    Depositor

    Piyush Jain

    Article

    Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis and 6xHis, MBP, TEV cleavage site

    Expression

    Bacterial

    Mutation

    R160E, F208W, N524V, D868V

    Promoter

    T7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mNeonGreen-peroxi

    Plasmid

    #137806

    Purpose

    Visualization of peroxisomes

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    SKL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMax_mOrange

    Plasmid

    #177827

    Purpose

    Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCS2-Tol1.zf1

    Plasmid

    #61388

    Purpose

    mRNA synthesis of tol1 transposase codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    tol1.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PCV-Cas9

    Plasmid

    #123643

    Purpose

    Expresses Cas9 with an N-terminal HUH-tag called PCV2

    Depositor

    Wendy Gordon

    Article

    Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

    Inserts

    PCV2 (ORF1 )
    Cas9
    

    Use

    Tags

    (EAAAK)8 linker and His6 + SUMO

    Expression

    Bacterial

    Mutation

    2-116

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pD454-SR mouse Alpha-synuclein

    Plasmid

    #89075

    Purpose

    bacterial expression of mouse Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. LRG (Lenti_sgRNA_EFS_GFP)

    Plasmid

    #65656

    Purpose

    Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

    Depositor

    Christopher Vakoc

    Article

    Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter-driven sgRNA expression and EFS promo…

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3 LIC cloning vector (6A)

    Plasmid

    #30124

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-UBR5 ∆HECT

    Plasmid

    #52051

    Purpose

    CMV driven mammalian expression of ligase-dead UBR5 with an N-termianl GFP fusion

    Depositor

    Darren Saunders

    Article

    Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

    Insert

    UBR5 ∆HECT (UBR5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C2769A

    Promoter

    CMV

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. cfSGFP2-col4a1

    Plasmid

    #160317

    Purpose

    cfSGFP2-tagged collagen4a1 for mammalian expression

    Depositor

    Ikuo Wada

    Article

    Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

    Insert

    COL4A1 (COL4A1 Human)
    

    Use

    Tags

    cfSGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET29b_1_anti-GFP-nanobody

    Plasmid

    #210394

    Purpose

    Express anti-GFP nanobody in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    1_anti-GFP-nanobody
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

    Plasmid

    #117484

    Purpose

    Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    SpyCatcher002-6aa-sfCherry2(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTol1-UAS:ChrimsonR-tdTomato

    Plasmid

    #124231

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    ChrimsonR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PRS_8-eGFP

    Plasmid

    #192589

    Purpose

    AAV-vector for fluorescent labeling of noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRSX8

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG-Synapto-pHluorin

    Plasmid

    #168510

    Purpose

    Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    Synaptophysin-pHluorin (Syp Mouse)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74010

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5xUAS
    Cas9
    Cre
    U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP-C1-A20

    Plasmid

    #22141

    Depositor

    Yihong Ye

    Article

    Li et al Biochim Biophys Acta. 2008 Jun . 1783(6)

    Insert

    A20 (TNFAIP3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74009

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5x UAS
    Cas9
    T2A
    GFP
    U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p6599 MSCV-IP N-HAonly FOSL1

    Plasmid

    #34897

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    FOSL1 (FOSL1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge