We narrowed to 3,688 results for: Kap

1. RelA RHD cFlag pcDNA3

   Plasmid

   #20015

   Depositor

   Stephen Smale

   Insert

   RelA (Rela Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Contains only the Rel Homology Domain of RelA. It…

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pJK651

   Plasmid

   #71702

   Purpose

   Produces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin reductase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK217

   Plasmid

   #72471

   Purpose

   Produces Acetobacter aceti 1023 citrate synthase with N-terminal His6 tag (H6AaAarA)

   Depositor

   T. J. Kappock

   Insert

   citrate synthase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK551

   Plasmid

   #72425

   Purpose

   Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase, N-terminal His6 tag (H6AaUctD)

   Depositor

   T. J. Kappock

   Insert

   putative CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK406

   Plasmid

   #72420

   Purpose

   Produces Acetobacter aceti 1023 Est05, C-terminal His6 tag

   Depositor

   T. J. Kappock

   Insert

   hypothetical protein
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   additional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…

   Promoter

   T7

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK369

   Plasmid

   #73594

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope

   Depositor

   T. J. Kappock

   Insert

   delta-12 fatty acid acetylenase
   

   Use

   Tags

   FLAG tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK545

   Plasmid

   #74225

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)

   Depositor

   T. J. Kappock

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   contains a silent mutation in Ala63 codon

   Promoter

   T7

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK139

   Plasmid

   #73315

   Purpose

   Pas2p peroxisomal membrane biogenesis protein

   Depositor

   T. J. Kappock

   Insert

   Pas2p peroxisomal membrane targeting protein
   

   Use

   Tags

   His6 and c-myc

   Expression

   Yeast

   Mutation

   Encodes a glycine-204 to serine mutation

   Promoter

   AOX1

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK267

   Plasmid

   #72432

   Purpose

   Acetobacter aceti 1023 gDNA

   Depositor

   T. J. Kappock

   Insert

   Acetobacter aceti 1023 gDNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   n/a

   Available since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pJK137

    Plasmid

    #73313

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tag

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Use

    Tags

    His6 and c-myc

    Expression

    Yeast

    Mutation

    synthetic gene

    Promoter

    5'-AOX1

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK361

    Plasmid

    #72403

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase, N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK108

    Plasmid

    #73312

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK368

    Plasmid

    #72407

    Purpose

    Produces Acetobacter aceti 1023 Est12

    Depositor

    T. J. Kappock

    Insert

    acyl-CoA thioesterase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal 15 residues MSETYGTGQKEQHC,…

    Promoter

    T7

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK692

    Plasmid

    #71643

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp/Ser38>Thr mutant (TdPurE2-H17W,S38T)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-17 to tryptophan, serine-38 to …

    Promoter

    T7

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK653

    Plasmid

    #71630

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr/Phe79>Trp mutant (TdPurE2-S38T,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to threonine, phenylalanine-79 …

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK654

    Plasmid

    #71631

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala/Phe79>Trp mutant (TdPurE2-S38A,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to alanine, phenylalanine-79 to…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK655

    Plasmid

    #71632

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp/Phe79>Trp mutant (TdPurE2-S38D,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to aspartate, phenylalanine-79 …

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK656

    Plasmid

    #71633

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val/Phe79>Trp mutant (TdPurE2-S38V,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to valine, phenylalanine-79 to …

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK659

    Plasmid

    #71634

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys/Phe79>Trp mutant (TdPurE2-S38C,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to cysteine, phenylalanine-79 t…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK660

    Plasmid

    #71635

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes lysine-41 to methionine, phenylalanine-79…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJK661

    Plasmid

    #71636

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg/Phe79>Trp mutant (TdPurE2-K41R,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes lysine-41 to arginine, phenylalanine-79 t…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJK662

    Plasmid

    #71637

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp/Cys87>Ser mutant (TdPurE2-F79W,C87S)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes phenylalanine-79 to tryptophan, cysteine-…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJK689

    Plasmid

    #71642

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn/Phe79>Trp mutant (TdPurE2-S38N,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to asparagine, phenylalanine-79…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJK697

    Plasmid

    #71646

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp/Ser38>Thr mutant (TdPurE2-M15W,S38T)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes methionine-15 to tryptophan, serine-38 to…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK582

    Plasmid

    #71618

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala mutant (AaPurE1-S57A)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-57 to alanine, additional N-termin…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJK583

    Plasmid

    #71619

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys mutant (AaPurE1-S57C)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-57 to cysteine, additional N-termi…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJK584

    Plasmid

    #71620

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr mutant (AaPurE1-S57T)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-57 to threonine, additional N-term…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJK608

    Plasmid

    #71621

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp mutant (AaPurE1-S57D)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-57 to aspartate, additional N-term…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJK677

    Plasmid

    #71623

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val mutant (AaPurE1-S57V)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-57 to valine, additional N-termina…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJK686

    Plasmid

    #71624

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asn mutant (AaPurE1-S57N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-57 to asparagine, additional N-ter…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJK657

    Plasmid

    #71647

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Ala/Phe79>Trp mutant (TdPurE2-H40A,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-40 to alanine, phenylalanine-79…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJK658

    Plasmid

    #71648

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Asn/Phe79>Trp mutant (TdPurE2-H40N,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-40 to asparagine, phenylalanine…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJK538

    Plasmid

    #72445

    Purpose

    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJK640

    Plasmid

    #72467

    Purpose

    Encodes Escherichia coli evgA

    Depositor

    T. J. Kappock

    Insert

    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJK641

    Plasmid

    #72468

    Purpose

    Encodes Escherichia coli evgA (Plac noncoding)

    Depositor

    T. J. Kappock

    Insert

    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJK646

    Plasmid

    #72469

    Purpose

    Encodes Escherichia coli evgA (Plac coding)

    Depositor

    T. J. Kappock

    Insert

    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJK581

    Plasmid

    #71695

    Purpose

    Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag and Cys35>Ser mutant (AaTrxAH6-C35S)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes cysteine-35 to serine

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJK558

    Plasmid

    #71704

    Purpose

    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with Cys138>Ser mutant (AaTrxB1-C138S)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin reductase 1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes cysteine-138 to serine

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJK505

    Plasmid

    #71594

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, additiona…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJK506

    Plasmid

    #71596

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-165 to phenylalanine, addition…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDC10

    Plasmid

    #75077

    Purpose

    Produces Escherichia coli glycinamide ribonucleotide synthetase (H6EcPurD)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Insert

    phosphoribosylglycinamide synthetase (purD Escherichia coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    adds unique SacII site at Thr95-Ala96 by a silent…

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJK131

    Plasmid

    #71548

    Purpose

    Produces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    Alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGWF1

    Plasmid

    #18856

    Depositor

    Wolf Frommer

    Article

    Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

    Type

    Empty backbone

    Use

    Tags

    CFP and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pJK611

    Plasmid

    #72532

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJK626

    Plasmid

    #72461

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to cysteine, tyrosine-…

    Promoter

    T7

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pJK691

    Plasmid

    #72381

    Purpose

    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)

    Depositor

    T. J. Kappock

    Insert

    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. pJK292

    Plasmid

    #73371

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed histidine-59 to asparagine and histidine-…

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pJK293

    Plasmid

    #73372

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed histidine-59 to phenylalanine and histidi…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pJK294

    Plasmid

    #73373

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed histidine-59 to aspartate and histidine-8…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pJK528

    Plasmid

    #71625

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met105>Ser/Ile109>Trp mutant (TdPurE2-M105S,I109W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes methionine-105 to serine, isoleucine-109 …

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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