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Plasmid#123741PurposeEncodes cAMP reporter Flamindo2 as a Type 3 part to be used in the MTK systemDepositorInsertFlamindo2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-GBP
Plasmid#67672PurposeMultisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)DepositorInsertanti-GFP nanobody
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (1-1899)
Plasmid#170143PurposeExpresses residues 1-1899 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationC-terminal truncation of residues 1900-2997PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (787-1899)
Plasmid#170145PurposeExpresses residues 787-1899 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationN-terminal truncation of residues 1-786; C-termin…PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (787-2138)
Plasmid#170146PurposeExpresses residues 787-2138 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationN-terminal truncation of residues 1-786; C-termin…PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (1-1547)
Plasmid#170144PurposeExpresses residues 1-1547 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationC-terminal truncation of residues 1548-2997PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (936-2997)
Plasmid#170147PurposeExpresses residues 936-2997 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationN-terminal truncation of residues 1-935PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK1_022
Plasmid#123684PurposeEncodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS-A4 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-ML1N*2(7Q)
Plasmid#67679PurposeMultisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)DepositorInsertML1N*2(7Q)
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-synPCB2.0
Plasmid#139477PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
UseTagsExpressionMammalianMutationV413LPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
JPF0510
Plasmid#124001PurposeEncodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_014
Plasmid#123929PurposeEncodes the BxBI attB GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInsertBxBIattB destination vector
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK678_002
Plasmid#123923PurposeEncodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK systemDepositorInsertBAC-AmpR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_024
Plasmid#123686PurposeEncodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L2-C3 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-mCitrine
Plasmid#61676PurposeSingle mCitrine-labeled, truncated kinesin-1DepositorInsertKHC(1-560)-mCitrine (Kif5c Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sha2Cas9
Plasmid#192140PurposeExpresses Sha2Cas9, and cloning backbone for sgRNADepositorInsertSha2Cas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpeCas9
Plasmid#192139PurposeExpresses SpeCas9, and cloning backbone for sgRNADepositorInsertSpeCas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK1_023
Plasmid#123685PurposeEncodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L1- B1 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK2_008
Plasmid#123703PurposeEncodes human pUbc as a Type 2 part to be used in the MTK systemDepositorInsertphUbC
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0512
Plasmid#124003PurposeEncodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_spacer-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only