We narrowed to 3,870 results for: fas

1. L2E-2

   Plasmid

   #21194

   Depositor

   Paul Khavari

   Article

   Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

   Insert

   Mek2 (Esr1, MAP2K2 Mouse, Human)
   

   Use

   Retroviral

   Mutation

   deletion of residues EQQKKRLE, S222D S226D in Me…

   Available Since

   July 24, 2009

   Availability

   Academic Institutions and Nonprofits only

2. mycUb-25aa

   Plasmid

   #20256

   Depositor

   Nico Dantuma

   Article

   Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

   Insert

   Ubiquitin-25 amino acid extension (RBX1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Ubiquitin fused to N-terminus of GFP. Glycine 76 …

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

3. mycUb-20aa

   Plasmid

   #20255

   Depositor

   Nico Dantuma

   Article

   Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

   Insert

   Ubiquitin-20 amino acid extension (RBX1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Ubiquitin fused to N-terminus of GFP. Glycine 76 …

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

5. GFP-Ub

   Plasmid

   #11928

   Purpose

   Used for in vivo monitoring of ubiquitin localization.

   Depositor

   Nico Dantuma

   Article

   Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

   Insert

   Ubiquitin (UBC Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9n(BB) (PX460)

   Plasmid

   #48873

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2.2

   Plasmid

   #34885

   Depositor

   Irvin Chen

   Article

   Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

   Insert

   Sindbis virus envelope protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   SGN UTR in E1 domain

   Promoter

   CMV

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. UbG76V-eGFP-V5His_20aa

   Plasmid

   #23970

   Depositor

   Nico Dantuma

   Article

   Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

   Insert

   Ub-G76V-eGFP-V5HIS_20aa
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   G76V mutation in Ubiquitin. Ub fused to N-termin…

   Available Since

   March 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJC-20xUAS-TALE3-VP64-P10

   Plasmid

   #104607

   Purpose

   Expresses TALE3 under UAS control

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   TALE3
   

   Use

   TALEN

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJC-20xUAS-TALE4-VP64-P10

    Plasmid

    #104608

    Purpose

    Expresses TALE4 under UAS control

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    TALE4
    

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

    Plasmid

    #41863

    Purpose

    This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX429 SpCas9 N863A nickase

    Plasmid

    #68706

    Purpose

    SpCas9 N863A nickase

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    SpCas9 N863A nicking mutant
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. human p53-(1-393)-L344P

    Plasmid

    #24862

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Leucine 344 mutated to Proline

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

14. human p53-(1-393)-N268D

    Plasmid

    #24861

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Asparagine 268 mutated to Aspartate

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

15. human p53-Δ(325-355)

    Plasmid

    #24871

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Deletion of aa 325-355

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

16. human p53-(310-360)

    Plasmid

    #24872

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains only the dimeric oligomerization domain …

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pGENE:EFF-1::mCherry::V5

    Plasmid

    #133020

    Purpose

    EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

    Insert

    EFF-1::mCherry::V5
    

    Tags

    V5; mCherry

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. human p53-(1-393)-M340Q-L344R

    Plasmid

    #24863

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Methionine 340 mutated to Glutamine Leucine 344 …

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

19. PX544 SpCas9(del1098-1368)

    Plasmid

    #58889

    Purpose

    Truncated form of SpCas9 without C' domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 1098-1368

    Available Since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pre_IV_CS6_4323_v1

    Plasmid

    #126859

    Purpose

    4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    IV_CS6_4323_v1
    

    Use

    Synthetic Biology

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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