We narrowed to 10,167 results for: otos

1. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

   Plasmid

   #177344

   Purpose

   AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   dead hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3x GCN4 peptide, 3xHA, and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   Tetracycline-dependent promoters

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-CMV-scFVDnmt3A_bGHpA

   Plasmid

   #177349

   Purpose

   AAV expression of scFV-fused catalytic domain of Dnmt3a for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   598–908 aa of mouse Dnmt3a

   Promoter

   CMV

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CMV-scFVDnmt3L_bGHpA

   Plasmid

   #177350

   Purpose

   AAV expression of scFV-fused C terminal domain of Dnmt3l for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   194–415 aa

   Promoter

   CMV

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-scFVtet2_bGHpA

   Plasmid

   #177353

   Purpose

   AAV expression of scFV-fused catalytic domain of TET2 from Synapisin promoter for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   Tet Methylcytosine Dioxygenase 2 (TET2 Human)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   Catalytic domains of human TET2 (1129–1936 and 14…

   Promoter

   human Synapsine promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-TRE-scFVDnmt3L_bGHpA

   Plasmid

   #177356

   Purpose

   AAV expression of scFV-fused C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   194–415 aa

   Promoter

   CMV promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-TRE-scFVtet2_bGHpA

   Plasmid

   #177358

   Purpose

   AAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   Tet Methylcytosine Dioxygenase 2 (TET2 Human)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   Catalytic domains of human TET2 (1129–1936 and 14…

   Promoter

   Tetracycline-dependent promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

   Plasmid

   #195048

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

   Plasmid

   #195047

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

   Plasmid

   #195046

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-Hb9-CD14

    Plasmid

    #204344

    Purpose

    Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    human CD14 (CD14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Hb9 (Mnx1) 9kb promoter fragment

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-tetO-NB

    Plasmid

    #197092

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEB multi-Hyg Human collagen type IV alpha 4 chain (COL4A4)

    Plasmid

    #229771

    Purpose

    3xFlag-tagged human Col4A4 to be used with N- or C-tagged LgBiT Col4A5 and SmBiT Col4A3 in Nanobit assay system

    Depositor

    Hirofumi Kai

    Article

    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

    Insert

    Human collagen type IV alpha 4 chain (COL4A4) (COL4A4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-CMV-scFVDnmt3AL_bGHpA

    Plasmid

    #177347

    Purpose

    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    

    Use

    AAV

    Tags

    C-terminal domain of mouse Dnmt3l (194–415 aa), P…

    Expression

    Mammalian

    Mutation

    598–908 aa of mouse Dnmt3a

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-CMV-scFVtet1_bGHpA

    Plasmid

    #177351

    Purpose

    AAV expression of scFV-fused catalytic domain of TET1 for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    N terminal domain of human Tet1

    Promoter

    CMV promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid

    #60600

    Purpose

    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    CRISPR Cas9 D10A
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Changed Asp 10 to Ala, Codon usage optimized for …

    Promoter

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-hSyn-scFVDnmt3AL_bGHpA

    Plasmid

    #177348

    Purpose

    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from Synapisin promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    

    Use

    AAV

    Tags

    C-terminal domain of mouse Dnmt3l (194–415 aa), P…

    Expression

    Mammalian

    Mutation

    598–908 aa of mouse Dnmt3a

    Promoter

    human Synapsine

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-TRE-scFVDnmt3AL_bGHpA

    Plasmid

    #177354

    Purpose

    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    

    Use

    AAV

    Tags

    C-terminal domain of mouse Dnmt3l (194–415 aa), P…

    Expression

    Mammalian

    Mutation

    598–908 aa of mouse Dnmt3a

    Promoter

    Tetracycline-dependent promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-TRE-scFVDnmt3A_bGHpA

    Plasmid

    #177355

    Purpose

    AAV expression of scFV-fused catalytic domain of Dnmt3a from doxycycine-inducible promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    598–908 aa of mouse Dnmt3a

    Promoter

    Tetracycline-dependent promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-TRE-scFVtet1_bGHpA

    Plasmid

    #177357

    Purpose

    AAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    N terminal domain of human Tet1

    Promoter

    Tetracycline-dependent promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTW5126

    Plasmid

    #129732

    Purpose

    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

    Insert

    eatA-DUF::His8
    

    Use

    Tags

    9His tag

    Expression

    Bacterial

    Mutation

    replaces the domain of unknown function correspon…

    Promoter

    araBAD

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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