We narrowed to 2,317 results for: pET28

1. WT N-His Ana GvpC in pET28a

   Plasmid

   #153294

   Purpose

   Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Gas vesicle protein C (N-terminal his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added a hexahistidine tag after the methionine at…

   Promoter

   T7

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-sfGFP[D134TAG+N150TAA]

   Plasmid

   #164582

   Purpose

   superfolder GFP with D134TAG and N150TAA mutations

   Depositor

   Ryan Mehl

   Article

   Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

   Insert

   sfGFP-134TAG+150TAA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D134TAG + N150TAA Mutations

   Promoter

   T7

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pF714-pET28a-Hs-FL-METTL20-NHis wt

   Plasmid

   #85115

   Purpose

   expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human full-length METTL20 (METTL20 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pET28a(+) 6xHis-TEV-IFIT5

   Plasmid

   #53560

   Purpose

   bacterial expression of IFIT5

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT5 (IFIT5 Human)
   

   Use

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pET28a-Hisx6-Sirt6-H133Y

   Plasmid

   #20279

   Depositor

   Leonard Guarente

   Article

   Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

   Insert

   Sirt6 (Sirt6 Mouse)
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Histidine 133 to Tyrosine

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pET28-Strep-TEV-Nsp14

   Plasmid

   #154955

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Strep-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET28-His6-Ubc9-C93A

   Plasmid

   #133910

   Purpose

   Bacterial expression of His6-Ubc9-C93A

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Cysteine 93 to Alanine

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-Gluc-EL222-his

   Plasmid

   #172097

   Purpose

   Expresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteria

   Depositor

   Avi Schroeder

   Article

   Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

   Insert

   Gaussia Luciferase - EL222
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gluc mutations - M43L, M110L

   Promoter

   T7

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28b(+)_S. cerevisiae Hbs1(1-611)

   Plasmid

   #39336

   Depositor

   SeWon Suh

   Insert

   Elongation factor 1 alpha-like protein
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pET28-sfGFP-SpyTag003 Loop A

    Plasmid

    #171776

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop A
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues V…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-MRPP3-myc-His

    Plasmid

    #67864

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP3 (KIAA0391 Human)
    

    Use

    Tags

    His and myc

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-MBP-(CBX1)3-eReader

    Plasmid

    #135090

    Purpose

    Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered x3
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-His6-Ubc9-C138A

    Plasmid

    #133911

    Purpose

    Bacterial expression of His6-Ubc9-C138A

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Use

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    Cysteine 138 to Alanine

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-TAF4(827-909)

    Plasmid

    #105620

    Purpose

    bacterially express TAF4 HFD

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF4-HFD(827-909) (Taf4 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28A-p53 (1-73) (P12A P13A)

    Plasmid

    #62066

    Purpose

    Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P12A, P13A

    Promoter

    T7

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-Hisx6-Sirt6-S56A

    Plasmid

    #20278

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Serine 56 to Alanine

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pET28b-NmCas9-Flag H588A

    Plasmid

    #109032

    Purpose

    H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28 His-SUMO-CHMP2B

    Plasmid

    #232018

    Purpose

    Bacterial expression of His and SUMO tagged CHMP2B

    Depositor

    Franz-Ulrich Hartl

    Insert

    CHMP2B (CHMP2B Human)
    

    Use

    Tags

    6x His, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-His6-UROD-N367A

    Plasmid

    #236740

    Purpose

    Protein overexpression of human uroporphyrinogen decarboxylase N367A

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    Uroporphyrinogen decarboxylase human N367A (UROD Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N367A

    Promoter

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-His6-GFP-UROD

    Plasmid

    #236741

    Purpose

    Protein overexpression of eGFP-human uroporphyrinogen decarboxylase

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    eGFP-Uroporphyrinogen decarboxylase human (UROD Synthetic, Human)
    

    Use

    Tags

    His6 and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge