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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,288 results for: crispr

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Showing: 8781 - 8800 of 25288 results

  1. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Publication
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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  4. pCAG-T3-st1CAS9-pA

    Plasmid
    #131542
    Purpose
    Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized st1Cas9
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131542-plasmid-map-sequence-id-262465

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  5. pYPQ165

    Plasmid
    #109327
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter
    Depositor
    Yiping Qi
    Publication
    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.
    Insert
    zCas9
    Use
    CRISPR; Gateway compatible zcas9 entry clone
    Tags
    3x FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    Promoter EC1.2 enhancer fused to EC1.1 promoter
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    109327-plasmid-map-sequence-id-210214

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  6. B52 + TIMELESS sgSTOP

    Plasmid
    #100713
    Purpose
    B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Publication
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgSTOP targeting TIMELESS (cloned using BbsI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100713-plasmid-map-sequence-id-193647

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  7. B52 + PARP4 sgSTOP

    Plasmid
    #100711
    Purpose
    B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Publication
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgSTOP targeting PARP4 (cloned using BbsI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100711-plasmid-map-sequence-id-193646

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  16. ICBE

    Plasmid
    #247331
    Purpose
    Vector encoding human codon-optimized engineered DelIscB nickase fusion with APOBEC3A and UGI driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yong Tian
    Insert
    pCAG_enDelIscB(D60A)-hAPOBEC3A(W98Y/W104A/Y130F)-2×UGI_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
    Expression
    Mammalian
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247331-plasmid-map-sequence-id-496500

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  17. pJAT43

    Plasmid
    #204299
    Purpose
    For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ie1-EGFP
    Use
    CRISPR
    Promoter
    ie1
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204299-plasmid-map-sequence-id-406188

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Showing: 8781 - 8800 of 25288 results