We narrowed to 166,303 results for: addgene

1. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

   Plasmid

   #207760

   Purpose

   MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pNA0304

   Plasmid

   #165612

   Purpose

   Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   NotI site replaced the CAN1 spacer in parent vect…

   Promoter

   SNR52

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMKO shRNA Bad

   Plasmid

   #17237

   Depositor

   Joan Brugge

   Article

   Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

   Insert

   Bad shRNA (BAD Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pMXs-ms-Sox1

   Plasmid

   #50778

   Purpose

   retroviral expression of mouse Sox1

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

   Insert

   Sox1 (Sox1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. MK13_HUMAN_D0

   Plasmid

   #79693

   Purpose

   This plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK13
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-EF1a-FRT-EBFP-tTAn

   Plasmid

   #172127

   Purpose

   Expression of EBFP and tTA-InteinN in Flp recombinase positive cells

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   EBFP, tTAn-InteinN
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-sgRNA_Ago2_3

   Plasmid

   #73531

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA2_IL1RN

   Plasmid

   #64142

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA4_IL1RN

   Plasmid

   #64152

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA4_IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. SOD1_pCSdest

    Plasmid

    #53879

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    SOD1 (SOD1 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    Sept. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. XLone-BSD ID2

    Plasmid

    #179513

    Purpose

    Tunable and temporal expression control of ID2

    Depositor

    Xiaoping Bao

    Article

    Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

    Insert

    ID2 (ID2 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3GS Promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-p19Ink4d-IRES-GFP

    Plasmid

    #52221

    Purpose

    retroviral bicistronic expression of p19Ink4d and GFP

    Depositor

    Martine Roussel

    Article

    Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub

    Insert

    Ink4d (Cdkn2d Mouse)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

13. lentiCRISPR.sgKras.9

    Plasmid

    #91894

    Purpose

    sgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.

    Depositor

    Wen Xue

    Article

    Mou et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    Kras sgRNA
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX458-sgRNA_Ago2_4

    Plasmid

    #73532

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLIK TT 3xFLAG ID2 neo

    Plasmid

    #98393

    Purpose

    Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    ID2 (ID2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MIS18A_pLENTI-CAG-IRES-GFP

    Plasmid

    #176996

    Purpose

    Mammalian lentiviral expression vector encoding MIS18A

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    MIS18A (MIS18A Human)
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. VRK3_HUMAN_D0

    Plasmid

    #79681

    Purpose

    This plasmid encodes the kinase domain of VRK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    VRK3 (VRK3 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-VTKS5-TVA-eGFP-N2cG

    Plasmid

    #175440

    Purpose

    Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

    Depositor

    Gordon Fishell

    Article

    Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

    Insert

    TVA-P2A-eGFP-P2A-N2cG
    

    Use

    AAV and Cre/Lox

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Zebrafish-gRNA-0005

    Plasmid

    #42245

    Purpose

    gRNA targeted to zebrafish gene gsk3b

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-gsk3b (gsk3b Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

20. FB007

    Plasmid

    #119705

    Purpose

    Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Promoter gpdA
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Promoter

    gpdA

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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