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pcDNA3-Flag-SP1

Plasmid

#232649

Purpose

expresses SP1 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

SP1 (SP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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Akaluc-N1

Plasmid

#120371

Purpose

Akaluc luciferase mammalian expression in -N1 backbone

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Akaluc (human optimized)

Expression

Mammalian

Mutation

Human codon optimized

Promoter

CMV

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pZR064_CROPseq-sgCD4-CRISPRi

Plasmid

#180270

Purpose

Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

CD4 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pC1-mitoRexYFP

Plasmid

#60246

Purpose

Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

Depositor

Vsevolod Belousov

Article

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

mitoRexYFP

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLVU/RED

Plasmid

#24178

Depositor

Lars Ittner

Article

Krupka et al Plasmid. 2010 May;63(3):155-60. doi: 10.

Insert

Chloramphenicol resistance gene (CmR) - ccdB gene

Use

Lentiviral; Gateway

Tags

DsRED

Expression

Mammalian

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV: Flag-Akaluc_SV40:PuroR

Plasmid

#183048

Purpose

Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

AkaLuc

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD1

Plasmid

#153116

Purpose

Expression of Flag tagged human FOXD1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD1 (FOXD1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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VPS13A^mCherry

Plasmid

#118758

Purpose

VPS13A internally tagged with mCherry at residue 1372.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13A (VPS13A Human)

Tags

mCherry inserted at position 1372

Expression

Mammalian

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CDK7 HA (P#633)

Plasmid

#14647

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk7 (CDK7 Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pMpGWB103

Plasmid

#68557

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRT802

Plasmid

#68113

Purpose

Integration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.

Depositor

Maggie Smith

Article

Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

Inserts

aac3(IV)
aph11
oriT
attP
int

Use

Streptomyces integrating vector

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-BCL6-IRES-GFP

Plasmid

#31391

Depositor

Hilda Ye

Article

Yu et al Blood. 2005 Feb 15. 105(4):1777-84.

Insert

B-cell lymphoma 6 (BCL6 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-pDEST-N

Plasmid

#118368

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cherry_Nanotag_117

Plasmid

#130552

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-mTurquoise

Plasmid

#193092

Purpose

constitutive expression of mTurquoise in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mTurquoise

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_89

Plasmid

#130538

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_32_neg_Control

Plasmid

#130563

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_18

Plasmid

#130519

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Cerulean_Nanotag_91_positive_Control

Plasmid

#130572

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC3

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pZR060_CROPseq-sgCtrl-CRISPRi

Plasmid

#180269

Purpose

Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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