We narrowed to 9,493 results for: pho;

1. pAf-CRISPR-ctfR1

   Plasmid

   #207992

   Purpose

   CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

   Insert

   ctfR1
   

   Use

   CRISPR

   Promoter

   Aspergillus flavus U6

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG302-CPL2-ZF

   Plasmid

   #200918

   Purpose

   Express CPL2-ZF108 in Arabidopsis target FWA gene

   Depositor

   Steven Jacobsen

   Article

   Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

   Insert

   CPL2 (CPL2 Mustard Weed)
   

   Expression

   Plant

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-LS

   Plasmid

   #205438

   Purpose

   Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes

   Depositor

   Masamichi Kamihira

   Article

   Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

   Inserts

   Gaussia luciferase gene
   spectinomycin resistance gene
   

   Use

   Cre/Lox and Luciferase; Chlamydomonas expression

   Promoter

   Heat shock protein 70A (HSP70A)/ribulose bisphosp…

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCC_162 SpCas9 KES(1107-1109)GG

   Plasmid

   #179526

   Purpose

   For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 KES(1107-1109)GG
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   replaced KES (residues 1107-1109) with GG

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPQD0CM0065

   Plasmid

   #177019

   Purpose

   Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   1-deoxy-D-xylulose 5-phosphate synthase
   

   Use

   Synthetic Biology

   Mutation

   transit peptide removed, amplified from cDNA, con…

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM-HE-bPAC(R278A)-Myc-eYFP

   Plasmid

   #165488

   Purpose

   Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   bPAC(R278A)-Myc-eYFP
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   EYFP and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTF101.1gw3

   Plasmid

   #172181

   Purpose

   A binary plasmid for gateway 3-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

   Depositor

   Kan Wang

   Article

   Paz et al Plant Cell Rep. 2006 Mar;25(3):206-13. d

   Insert

   attR3-CmR-ccdB-attR4
   

   Expression

   Plant

   Promoter

   lacUV5 promoter

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

   Plasmid

   #159852

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

   Plasmid

   #163927

   Purpose

   Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. D62_Ld_362720

    Plasmid

    #135875

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    membrane-bound acid phosphatase 2, putative
    

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. D46_Ld_362700

    Plasmid

    #135860

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    membrane-bound acid phosphatase precursor
    

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. D79_Ld_366740

    Plasmid

    #135892

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    histidine secretory acid phosphatase, putative
    

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-Lact-C2

    Plasmid

    #109553

    Purpose

    Multisite gateway vector for 3' tagging with the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8).
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ad-MKP-3 153-381

    Plasmid

    #131524

    Purpose

    Expresses MKP-3 153-381 in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

    Insert

    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Mutation

    only reserved amino acid 153-381

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Unc5c.a-AP-His

    Plasmid

    #72054

    Purpose

    Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5c.a (Unc5c Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHBS1039 TDP43 RRM-GFP 2xΦ

    Plasmid

    #107806

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All hydrophobics combined to two clusters in LLPS…

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHBS1038 TDP43 RRM-GFP 4xΦ

    Plasmid

    #107805

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All hydrophobics combined to four clusters in LLP…

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHBS1037 TDP43 RRM-GFP 6xΦ

    Plasmid

    #107804

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All hydrophobics combined to six clusters in LLPS…

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_NCF1-2/2

    Plasmid

    #91492

    Purpose

    Protein expression and purification of human SH3 domain construct NCF1-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCF1-2/2 (NCF1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_NCF1-1/2

    Plasmid

    #91305

    Purpose

    Protein expression and purification of human SH3 domain construct NCF1-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCF1-1/2 (NCF1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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