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pFB-MeCP2Y.3 (pc1575)

Plasmid

#211717

Purpose

Expresses MeCP2 (aa1-163) YFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa1-163

Tags

YFP

Expression

Insect

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2mR (pc2519)

Plasmid

#211716

Purpose

Expresses fl. MBD2 RFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Tags

RFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFBMBD4-C-GFP (pc1567)

Plasmid

#211714

Purpose

Expresses fl. MBD4 GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD4

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFBMBD3-C-GFP (pc1566)

Plasmid

#211713

Purpose

Expresses fl. MBD3 GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD3

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFBMBD1a-C-GFP (pc1564)

Plasmid

#211711

Purpose

Expresses fl. MBD1 GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD1

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMeCP2R.9 (pc2812)

Plasmid

#211574

Purpose

Expresses MeCP2 (aa163-309) GFP tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa163-309

Tags

RFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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IAA17-4flag-HIS5

Plasmid

#207015

Purpose

For ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-HIS5

Plasmid

#207014

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-MET15

Plasmid

#207013

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-URA3

Plasmid

#207012

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-sp6-kcnk4b

Plasmid

#213123

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk4b (LOC101882857 Zebrafish)

Use

Cloning

Mutation

Stop codon removed

Promoter

T7

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS2-4

Plasmid

#184730

Purpose

COX2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-homo_PLA2G4A-1

Plasmid

#184735

Purpose

cPLA2-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human cPLA2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-homo_PLA2G4A-2

Plasmid

#184736

Purpose

cPLA2-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human cPLA2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-OptoSTIM1(CRY2clust)

Plasmid

#184715

Purpose

OptoSTIM1(CRY2clust)

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

OptoSTIM1

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-EBFP2-OptoSTIM1(CRY2clust)

Plasmid

#184716

Purpose

OptoSTIM1(CRY2clust)

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

OptoSTIM1

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-PTGER2

Plasmid

#184721

Purpose

Exogenous EP2

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

PTGER2 (PTGER2 Synthetic, Human)

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-bleo-COX1-4

Plasmid

#184722

Purpose

COX1-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX1 gene.

Use

CRISPR and Lentiviral

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-COX2-4

Plasmid

#184723

Purpose

COX2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX2 gene.

Use

CRISPR and Lentiviral

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2-bleo-PTGER2

Plasmid

#184724

Purpose

EP2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog EP2 gene.

Use

CRISPR and Lentiviral

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge