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mCherry pBSDONR P4r-P2

Plasmid

#121528

Purpose

For making C-terminal mCherry fusions using multisite Gateway cloning

Depositor

Roger Innes

Publication

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Insert

mCherry

Expression

Bacterial

Available since

Aug. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10_K139R

Plasmid

#127137

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K139R

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10_K138R

Plasmid

#127136

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K138R

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-SNAP-HCA_sensor

Plasmid

#127181

Purpose

Bacterial expression of the fusion protein used for sulfa-drugs sensing

Depositor

Kai Johnsson

Publication

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA

Tags

HisTag and StrepTag

Expression

Bacterial

Promoter

T7

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgPOLR2D

Plasmid

#125769

Purpose

constitutive expression of a guide RNA targeting human POLR2D (CRISPR positive control)

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgPOLR2D (POLR2D Human)

Use

CRISPR

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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FB010

Plasmid

#119708

Purpose

Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha1. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Publication

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

PtrpC:hph:Ttub

Use

Synthetic Biology

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1(406)

Plasmid

#122068

Purpose

Expressing ATG1

Depositor

Daniel Klionsky

Publication

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK jannaschii

Plasmid

#108948

Purpose

heterologous expression of MVK of M. jannaschii in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Tags

His6-Tag

Expression

Bacterial

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagA(R37P)

Plasmid

#99717

Purpose

Mammalian expression of Flag-RagA(R37P)

Depositor

David Sabatini, Kuang Shen

Publication

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Tags

Flag

Mutation

R37P

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-Myocardin

Plasmid

#100769

Purpose

Lentiviral expression of shRNA targeting MYOCD

Depositor

Joseph Gordon

Publication

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Lenti-sh-Myocardin

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.1.1

Plasmid

#97355

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP4.3.1

Plasmid

#98269

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.22.1

Plasmid

#98205

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.21.6

Plasmid

#98204

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.21.4

Plasmid

#98202

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.20.3

Plasmid

#98197

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.19.2

Plasmid

#98193

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.19.1

Plasmid

#98192

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.11.4

Plasmid

#97380

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.11.3

Plasmid

#97379

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge