We narrowed to 14,263 results for: crispr grnas

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CSAC-Crys

Plasmid

#164967

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2

Use

AAV

Tags

mCherry

Promoter

hU6, hSyn

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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CSAC-Bmal1

Plasmid

#168296

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgBmal1-1-hU6-sgBmal1-3

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mTfeb

Plasmid

#79006

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

sgTfeb (Tfeb Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDIRECT_22A

Plasmid

#91133

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

Plasmid

#212961

Purpose

Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

DR1_CasRx_backbone

Use

CRISPR and Lentiviral

Tags

TagRFP657

Expression

Mammalian

Promoter

U6

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

Plasmid

#84258

Purpose

Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgTRE3G
PYL1-VPR

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Promoter

CMV and mouse U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

Plasmid

#84260

Purpose

Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgSV40
PYL1-KRAB

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Promoter

CMV and mouse U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE Cacnb3-GFP KI

Plasmid

#139662

Purpose

Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDIRECT_25F

Plasmid

#91143

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA

Use

CRISPR

Expression

Plant

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDIRECT_26G

Plasmid

#91149

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #3

Plasmid

#139659

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLC-EGFP-RIP3

Plasmid

#75165

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIP3

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIP3 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #2

Plasmid

#139658

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLC-mCherry-MLKL

Plasmid

#75167

Purpose

LentiCRISPR-mCherry with sgRNA targeting human MLKL

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

MLKL sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDIRECT_21A

Plasmid

#91128

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

Plasmid

#160608

Purpose

TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

P35s:dCas9:SRDX:tNOS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_mup53_Ex5

Plasmid

#85535

Purpose

Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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px330-CD4

Plasmid

#136938

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting CD4 (CD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTE4254

Plasmid

#89050

Purpose

Expresses St chimeric gRNA and human codon-optimized StCas9

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

St chimeric gRNA
hStCas9

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_huBim_Ex3

Plasmid

#85532

Purpose

Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Bim

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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