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pDGE504

Plasmid

#153277

Purpose

Intermediate sgRNA array cloning vector position 2

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE508

Plasmid

#153280

Purpose

End-linker for cloning 2 sgRNA arrays into any recipient vector

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

linker

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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MTK5_012

Plasmid

#123856

Purpose

Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-A1 Insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_022

Plasmid

#123684

Purpose

Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-A4 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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aL-L3

Plasmid

#103881

Purpose

full-length human aL with moxsynGFP in betapropeller

Depositor

Timothy Springer

Article

Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi:

Insert

full-length human aL with moxsynGFP in betapropeller (ITGAL Human)

Tags

tension sensor module

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

Plasmid

#99221

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.1

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2sh-HA

Plasmid

#97072

Purpose

Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

HA

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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N-BLa 1.1

Plasmid

#88994

Purpose

BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.1 fusion protein

Tags

Flag

Expression

Bacterial

Promoter

araBAD

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAP3K2

Plasmid

#85652

Purpose

retroviral expression of human MAP3K2

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

MAP3K2 (MAP3K2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSPneogRNA241510+MT

Plasmid

#84292

Purpose

Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA

Use

CRISPR; Leishmania donovani

Promoter

L. donovani ribosome RNA promoter

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSico shSMYD3-C human

Plasmid

#85654

Purpose

expression of shRNA targeting human SMYD3

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

shRNA targeting 3'UTR of SMYD3 (SMYD3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

Plasmid

#83489

Purpose

cre-dependent PAK1-AID under EF1a promoter

Depositor

Dong Kong

Article

Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

Insert

PAK1-AID-ires-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-nArgBP2ΔNSE

Plasmid

#74515

Purpose

Express GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

nArgBP2delNSE (Sorbs2 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-S285A/T289A-3xFLAG

Plasmid

#69841

Purpose

This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S285A/T289A

Promoter

CMV

Available Since

Dec. 3, 2015

Availability

Academic Institutions and Nonprofits only

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P12-bio

Plasmid

#68516

Purpose

Expresses full-length P. vivax P12 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

P12

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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MSP1-bio

Plasmid

#68505

Purpose

Expresses full-length P. vivax MSP1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

MSP1

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-GW-V5-Hygro

Plasmid

#68265

Purpose

Constitutive selectable expression of C-term V5 tagged proteins

Depositor

Mitchell Guttman

Article

McHugh et al Nature. 2015 May 14;521(7551):232-6. doi

Type

Empty backbone

Tags

V5

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only
pMT001

Plasmid

#64758

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a V5 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pFvl160

Plasmid

#64753

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to a 3xT7 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-HMGB1-WT

Plasmid

#194561

Purpose

Dox-inducible Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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