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Showing: 2201 - 2250 of 2374 results

  10. pENTR-B/c

    Plasmid
    #227962
    Purpose
    Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka
    Insert
    B/c
    Use
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227962-plasmid-map-sequence-id-454806

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  11. pMDC32B-B/c

    Plasmid
    #227963
    Purpose
    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka
    Insert
    B/c
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227963-plasmid-map-sequence-id-454828

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  18. pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a

    Plasmid
    #213401
    Purpose
    Plant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursor
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:
    Insert
    Arabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    213401-plasmid-map-sequence-id-422351

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  37. pcDNA3-HCMVgO-sfGFP (TB40/E)

    Plasmid
    #136451
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein O (UL74 Human betaherpesvirus 5)
    Use
    Tags
    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136451-plasmid-map-sequence-id-271690

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  38. hGFAP-GFP

    Plasmid
    #40592
    Depositor
    Albee Messing
    Article
    Zhuo et al Dev Biol. 1997 Jul 1;187(1):36-42.
    Insert
    GFP-S65T
    Use
    Mouse Targeting
    Tags
    MP-1 fragment (mouse protamine 1 intron and polya…
    Expression
    Bacterial and Mammalian
    Mutation
    Construct contains a mutated, humanized hGFP(S65T…
    Promoter
    hGFAP promoter
    Available since
    Oct. 4, 2012
    Availability
    Academic Institutions and Nonprofits only
    40592-plasmid-map-sequence-id-240470

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  41. scFv-GCN4-DNMT3a(R887E)-DNMT3l

    Plasmid
    #154141
    Purpose
    Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.
    Depositor
    Albert Jeltsch
    Article
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Insert
    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    Use
    Tags
    HA and sfGFP
    Expression
    Mammalian
    Mutation
    R887E
    Promoter
    SFFV
    Available since
    Oct. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154141-plasmid-map-sequence-id-345617

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  43. pD3sE_SC12

    Plasmid
    #219720
    Purpose
    DV3 E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV3 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219720-plasmid-map-sequence-id-433924

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  44. pD4sE_SC12

    Plasmid
    #219721
    Purpose
    DV4 stable E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV4 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A259W, S262R, F279W, A280P, G29K, T33V, A2…
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219721-plasmid-map-sequence-id-433923

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  45. pD1sE_SC12

    Plasmid
    #219718
    Purpose
    DV1 E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV1 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…
    Promoter
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    219718-plasmid-map-sequence-id-433425

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  46. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid
    #154140
    Purpose
    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.
    Depositor
    Albert Jeltsch
    Article
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Insert
    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    Use
    Tags
    HA and sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    Oct. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154140-plasmid-map-sequence-id-345614

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  47. pZVsE_SC12

    Plasmid
    #219722
    Purpose
    Zika virus stable E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    ZKV sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A264W, T267R, S286W, S287P, G29K, A25M
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219722-plasmid-map-sequence-id-433912

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  50. pD2sE_SC12

    Plasmid
    #219719
    Purpose
    DV2 stable E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV2 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A259W, T262R, F279W, T280P, S29K, T33V, A2…
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219719-plasmid-map-sequence-id-433926

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Showing: 2201 - 2250 of 2374 results