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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,260 results for: 338

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Showing: 881 - 900 of 6260 results

  1. 8xNFAT-ZsG-muhCD4

    Plasmid
    #162745
    Purpose
    NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene
    Depositor
    Maki Nakayama
    Article
    Landry et al Front Endocrinol (Lausanne). 2021 Mar 25
    Insert
    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    Human CD4 gene with glutamine to tyrosine at posi…
    Available Since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    162745-plasmid-map-sequence-id-331322

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  5. MCHR2-DuET

    Plasmid
    #213338
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MCHR2 (MCHR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213338-plasmid-map-sequence-id-430867

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  7. TFORF2596

    Plasmid
    #142338
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RORB (RORB Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142338-plasmid-map-sequence-id-397178

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  9. Epac-S-H74

    Plasmid
    #170338
    Purpose
    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2011 Apr 29;6(4):e19170. doi:
    Insert
    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170338-plasmid-map-sequence-id-335510

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  10. TFRC-His-bio

    Plasmid
    #52338
    Purpose
    Expresses full-length Transferrin receptor protein 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TFRC (TFRC Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52338-plasmid-map-sequence-id-81059

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  11. TFORF2108

    Plasmid
    #143338
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    L3MBTL4 (L3MBTL4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143338-plasmid-map-sequence-id-400322

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  14. LSB-hsa-miR-27a-3p

    Plasmid
    #103381
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-27a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-27a-3p target (MIR27A Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103381-plasmid-map-sequence-id-195811

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  19. LSB-hsa-miR-296-3p

    Plasmid
    #103387
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-296-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-296-3p target (MIR296 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103387-plasmid-map-sequence-id-195823

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  20. LSB-hsa-miR-28-3p

    Plasmid
    #103385
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-28-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-28-3p target (MIR28 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103385-plasmid-map-sequence-id-195819

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Showing: 881 - 900 of 6260 results