We narrowed to 72,194 results for: LAS

1. pLV-EF1a-MinE-IRES-Blast

   Plasmid

   #213115

   Purpose

   Expression of untagged MinE in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE (minE )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2T-CMV-evoCjCas9-eAID-BlastR

   Plasmid

   #194062

   Purpose

   Mammalian expression of evoCjCas9 eAID

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-eAID
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLOC CMV-IRES-Dsred-blasticidin

   Plasmid

   #129428

   Purpose

   expression of Dsred-blasticidin under CMVpromoter -IRES

   Depositor

   Oskar Laur

   Insert

   Dsred-blasticidin
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-EF1a-MCS-hPOT1_IRES-blast

   Plasmid

   #166411

   Purpose

   express hPOT1 in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   hPOT1 (POT1 Human)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   ef-1a

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-AcGFP-Sec61-IRES-Blast

   Plasmid

   #154009

   Purpose

   Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   acGFP-Sec61 (SEC61B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

   Plasmid

   #71217

   Purpose

   dCas9 fused to human DNMT3B catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3B catalytic domain
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Equalizer-H plasmid with onboard mCherry cassette

   Plasmid

   #169733

   Purpose

   Equalizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2T-CMV-miniSdd6-BE4max-BlastR

   Plasmid

   #204850

   Purpose

   For cytosine base editing using miniSdd6 in mammalian cells

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd6-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV, SV40, EM7

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-IRES-Blast 3XFLAG-CHP1

   Plasmid

   #173166

   Purpose

   Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
   

   Use

   Retroviral

   Tags

   3XFLAG

   Expression

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ppk301-T2A-QF2 HDR plasmid

    Plasmid

    #130667

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    

    Use

    Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHage2-EF1a-mCherryTRF1-IRES-Blast

    Plasmid

    #182588

    Purpose

    expresses mcherry-trf1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Anti-LRP4 (cytoplasmic) [N164/6.1R]

    Plasmid

    #114542

    Purpose

    Mammalian Expression Plasmid of anti-LRP4 (cytoplasmic) (Mouse). Derived from hybridoma N164/6.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-LRP4 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nano-lantern-plasma membrane/pcDNA3

    Plasmid

    #51975

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    HIS and Lyn

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pLenti-EF1a-GFP-miRE_shRen-Blast

    Plasmid

    #135682

    Purpose

    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-FLAG-IRES-Blasticidin-hOCT4 WT

    Plasmid

    #198764

    Purpose

    Mammalian expression of hOCT4

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

    Insert

    hOCT4 WT (POU5F1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV+ plasmid with onboard mCherry cassette

    Plasmid

    #169741

    Purpose

    CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE N' EGFP NAP1 FL IRES Blast

    Plasmid

    #179100

    Purpose

    Expression of full length NAP1 cDNA

    Depositor

    Alicia Pickrell

    Article

    Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

    Insert

    Nak associated protein 1 (AZI2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2T-CMV-HNHx-ABEmax7.10HD-BlastR

    Plasmid

    #176477

    Purpose

    A-to-G base editor with alternative targeting scope

    Depositor

    Gerald Schwank

    Article

    Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

    Insert

    HNHx-ABEmax7.10HD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV 4xSTAR-mTFP-NLS-blast

    Plasmid

    #136257

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mTFP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXS-IRES-Blast POLE1 N-ISCmut

    Plasmid

    #160803

    Purpose

    Express N-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 N-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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