We narrowed to 6,591 results for: nls

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Promoter

MBP

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

Plasmid

#196702

Purpose

Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 wild type

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-EGFP-NLS-3XFLAG-V5

Plasmid

#196090

Purpose

Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

GFP

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Promoter

CAGGs

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Wild-type-ThermoCas9-3xNLS

Plasmid

#254685

Purpose

Expresses Wild-type ThermoCas9 with 3x NLS tags. Utilized for RNP transfection

Depositor

Hong Li

Article

Roth et al Nature. 2026 Apr 15. doi: 10.1038/s41586

Insert

Wildtype ThermoCas9

Tags

NLS, SV40 NLS, and nucleoplasmin NLS

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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Lenti-TurboCas-NLS-Blast

Plasmid

#232346

Purpose

Lentiviral expression vector for dCas9 fused to miniTurboNLS

Depositor

Ali Shilatifard

Article

Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA, miniTurbo, V5 and 3xSV40 NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC-SV40NLS-Pfv-Sapphire

Plasmid

#203653

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-NLS-actin-R62D

Plasmid

#118381

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

NLS and R62D

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-eeBxb1 (CJT555)

Plasmid

#248209

Purpose

eeBxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-eeBxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

eeBxb1(V74A, E229K, V375I)

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-XBP1 mNeonGreen NLS

Plasmid

#115968

Purpose

ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

X-box binding protein 1 (XBP1 Synthetic, Human)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEMAX-NLS-P2A_BLAST

Plasmid

#135355

Purpose

Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-2XNLS

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-ATF4 mScarlet NLS

Plasmid

#115969

Purpose

ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

activating transcription factor 4 (ATF4 Synthetic, Human)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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NLS-mCherry-LEXY (pDN122)

Plasmid

#72655

Purpose

Blue light-inducible nuclear export of mCherry

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

NLS-mCherry-LEXY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-NLS-BirA-2A-mCherry

Plasmid

#127781

Purpose

BirA construct in a PCI nls backbone driving mCherry activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

BirA

Use

Unspecified

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-NLS-MaSat ZF-18-mVenusN

Plasmid

#191392

Purpose

Zinc finger fused to N-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

Flag-NLS-MaSat ZF-18-mVenusN

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:GFPNLS-SV40pA

Plasmid

#155123

Purpose

Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NLS-GFP

Plasmid

#104061

Purpose

AAV expression of nuclear localized GFP with SV40 NLS

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.eB

Insert

GFP

Use

AAV

Promoter

CAG

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-NLS-miRFP670nano-miRFP670

Plasmid

#216131

Purpose

Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE

Use

Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP-3XNLS

Plasmid

#58466

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Utr230 with 3X glycine linker (UTRN Human)
3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-mCherry-NLS

Plasmid

#165442

Purpose

Expresses nuclear mCherry from CMV promoter

Depositor

Esteban Engel

Article

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

mCherry-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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