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SPLICS Po-ER Long P2A

Plasmid

#164113

Purpose

Detect the long Peroxisomes-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP Po-ER Long P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-ER Short P2A

Plasmid

#164114

Purpose

Detect the long Peroxisomes-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP Po-ER Short P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPgiCas13b

Plasmid

#184833

Purpose

Inducible expression of PgiCas13b nuclease in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Cas13b nuclease from Porphyromonas gingivalis

Use

CRISPR

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-FLAG-TAT-86-E

Plasmid

#35978

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein (tat Human Immunodeficiency Virus)

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

From the "E" HIV clade

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-5ptaseOCRL

Plasmid

#66836

Purpose

Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

5ptaseOCRL (OCRL Human)

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-Mt Short P2A

Plasmid

#164116

Purpose

Detect the short Peroxisomes-mitochondria contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Short P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-Lckg2a)

Plasmid

#177891

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A

Promoter

Polyhedrin

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-iSH2

Plasmid

#66839

Purpose

Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

iSH2

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.Luciferase.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169317

Purpose

Doxycycline-inducible expression of Luciferase cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-dTomato

Promoter

TRE

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHR CD19-PI3K CAR

Plasmid

#113018

Purpose

for lentivirus production of CD19-PI3K CAR

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P intracellular PI3k recruiting domain from CD19 (CD19 Human, Synthetic)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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mito-CIBN-GFP

Plasmid

#89480

Purpose

Encodes for a fusion protein between a mitochondrial anchor and the CIBN-GFP protein.

Depositor

Xavier Trepat Guixer

Article

Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

Insert

mito-CIBN-GFP (CIB1 Human, Mustard Weed)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCas9-NT

Plasmid

#235165

Purpose

Expresses the codon harmonized Streptococcus pyogenes cas9 gene and a NT sgRNA

Depositor

Constantinos Patinios

Article

Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

Insert

SpCas9

Expression

Bacterial

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCas12a-NT

Plasmid

#235167

Purpose

Expresses the Wild Type Francisella novicida cas12a gene and a NT crRNA

Depositor

Constantinos Patinios

Article

Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

Insert

FnCas12a

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.Luciferase.IRES.dTomato

Plasmid

#169307

Purpose

Constitutive overexpression of Luciferase cDNA with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-dTomato

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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GreenT-EC - Display - CyRFP1

Plasmid

#193943

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC

Tags

Igk_secretion - HA-Flag and PDGFR

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a+GFP-CAMSAP3 CKK

Plasmid

#59033

Purpose

expresses CKK domain of CAMSAP3 in E. coli

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP3 CKK domain (CAMSAP3 Mouse)

Tags

His, Strep, TEV, and sfGFP

Expression

Bacterial

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
HSC1-GiP

Plasmid

#58254

Purpose

This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-1-101-FLAG

Plasmid

#35981

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV1 101 amino acids

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-AM

Plasmid

#206513

Purpose

AAV packaging vector containing AAV2 rep and AM cap and partial AAV2 p5 promoter

Depositor

Mark Kay

Article

Gonzalez-Sandoval et al Nat Commun. 2023 Apr 28;14(1):2448. doi:

Insert

AM capsid

Use

AAV

Promoter

p5

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GreenT-EC.b - Display - CyRFP1

Plasmid

#193944

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.b

Tags

Igk_secretion - HA-Flag and PDGFR

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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