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  5. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Expression
    Bacterial
    Promoter
    PBAD and PLtetO
    Available Since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61966-plasmid-map-sequence-id-101265

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  8. pGBW-m4046910

    Plasmid
    #145672
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145672-plasmid-map-sequence-id-297836

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  19. dFP-mini

    Plasmid
    #120543
    Purpose
    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.
    Depositor
    Brian Chow
    Article
    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/
    Insert
    de novo fluorescence-activating protein mini (dFP-mini)
    Tags
    6xHis - TEV cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    120543-plasmid-map-sequence-id-234163

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  20. pCB11

    Plasmid
    #201111
    Purpose
    pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.
    Depositor
    Mary Dunlop
    Article
    Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:
    Inserts
    CcaR
    tetA(C)
    sfGFP
    Expression
    Bacterial
    Mutation
    C-to-T transition at pos. no. 1049 of tetA(C) lea…
    Promoter
    J23100 and PcpcG2-172
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    201111-plasmid-map-sequence-id-403029

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Showing: 8981 - 9000 of 23077 results