We narrowed to 58,607 results for: plasmid
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Plasmid#220147PurposeExpresses VHL-BirA for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationcodon optimizedPromoterEF-1aAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PEmax-IRES-ZeoR
Plasmid#207353PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.DepositorInsertPrime editor max (PEmax) enzyme
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-PdCO-EGFP-WPRE (AAV1)
Viral Prep#198513-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_hSyn-PdCO-EGFP-WPRE (#198513). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-EGFP-WPRE plasmid DNA. Synapsin-driven expression of the optimized opsin PdCO in frame with EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5
Plasmid#208377PurposeEpisomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoringDepositorTypeEmpty backboneExpressionMammalianPromoterhuman CMVAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flpo (AAV2)
Viral Prep#55637-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-iGluSnFR.A184S (AAV9)
Viral Prep#106174-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.hSynapsin.SF-iGluSnFR.A184S (#106174). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.A184S plasmid DNA. hSynapsin driven SF-iGluSnFR.A184S glutamate sensor. The A184S mutant has a higher glutamate affinity. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-601_NY-ESO-1_TCR_GFP
Plasmid#207482PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertGFP, NY-ESO-1_TCR
UseCRISPRMutationPotential silent point mutation in GFP sequenceAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgRNA_expression_vector
Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneExpressionBacterial and MammalianPromoterU6Available SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCP-eN3x9
Plasmid#210706PurposeThis plasmid express EF1a promoter driven MCP-eN3x9 transactivation moduleDepositorInsertMCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 (AAV9)
Viral Prep#172923-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 (#172923). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 plasmid DNA. hSyn-driven expression of calcium sensor jGCaMP8f in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmRuby3Available SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pRTC2-puro
Plasmid#190567PurposeExpresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.DepositorInsertL1 (L1.3) with reporter cassette
ExpressionMammalianPromoterSV40Available SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hMLH1dn-Hygro
Plasmid#195512PurposeLentiviral expression plasmid of hMLH1dn with P2A-HygroR markerDepositorInserthMLH1dn-P2A-HygroR (MLH1 Human)
UseLentiviralTagsP2AExpressionMammalianMutationHuman MLH1 del754-756PromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_mPlum
Plasmid#177830PurposeHost Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)DepositorInsertmPlum
ExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-AID-CTERM-GFP
Plasmid#185778PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, AIDMutationnonePromoterPGKAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pegRNA-GFP>BFP_Y66H-NGG
Plasmid#185480PurposepegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.DepositorInsertGFP>BFP_Y66H-pegRNA
ExpressionMammalianPromoterU6Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVT36b
Plasmid#184437PurposeCRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Reporter-1
Plasmid#180218PurposeSurrogate reporter plasmid for the assay of targeted RNA editingDepositorInsertSurrogate reporter
UseLentiviralAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-eYFP (AAV1)
Viral Prep#117383-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-TRE-DIO-eYFP (#117383). In addition to the viral particles, you will also receive purified pAAV-TRE-DIO-eYFP plasmid DNA. Tet-inducible, Cre-dependent eYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTRETagseYFP (Tet-inducible, Cre-dependent)Available SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine (AAV5)
Viral Prep#50472-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine (#50472). In addition to the viral particles, you will also receive purified pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine plasmid DNA. GFAP-driven HA-tagged rM3D(Gs) and bicistronic mCitrine expression, for chemogenetic excitation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsmCitrineAvailable SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only