We narrowed to 10,242 results for: Uty
-
Plasmid#112146PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
ExpressionMammalianMutationnoneAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-IFNA-neo
Plasmid#225710PurposeThe pCDH-IFNA-neo plasmid is designed for the overexpression of human IFNα in mammalian cells. This plasmid was utilized to engineer A549 cells for stable IFNα overexpression.DepositorAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psMEK2
Plasmid#89363PurposeA lenti-viral vector expressing photoswitchable MEK2 in mammalian cellsDepositorInsertpsMEK2 (MAP2K2 Human, Synthetic)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianPromoterCMVAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40
Plasmid#194691PurposehSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_on-mEGFP
UseAAVTagsmEGFPPromoterhSyn1Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
ExpressionBacterialPromotertrcpAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DestFLAGHA_RBPMS2
Plasmid#65748Purposeconstitutively expresses FLAG-HA-tagged RBPMS2 in mammalian cellsDepositorAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mTurquoise2
Plasmid#111254PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasmDepositorInsertTorA(sp)-mTurquoise2
ExpressionBacterialMutationmTurquoise2 is codon optimized for expression in …PromoterPcAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATG-1929
Plasmid#108712PurposeCBR2opt in pF4Ag expression vector with T7 and CMV promoterDepositorInsertCBR2opt
ExpressionBacterial and MammalianMutationPromega used directed evolution to create CBR (Cl…PromoterT7, CMVAvailable SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-IGF2
Plasmid#133374PurposePiggybac vector for constitutive expressionDepositorInsertIGF2 (IGF2 Human)
TagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1PR1-DuET
Plasmid#213374PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast
Plasmid#197066PurposeDonor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBPDepositorInsertEncapsulin
UseCRISPRTagsFKBP and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B57
Plasmid#135516PurposeMammalian expression of myc-tagged HLA-B*57:01DepositorInsertHLA-B*57:1 (HLA-B Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-DIO-VGLUT2:miniSOG
Plasmid#214200PurposeFor Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)DepositorInsertSlc17a6 tagged with miniSOG and hemagglutinin
UseAAV and Cre/LoxTagsminiSOG (tagged with HA) is fused to the first in…ExpressionMammalianAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CHRM1-DuET
Plasmid#213207PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human CSF-1R-Y969F,S301-IRES—GFP
Plasmid#115524PurposeMammalian expression constructDepositorInserthuman CSF-1 receptor constitutively activated mutant (CSF1R Human)
UseRetroviralMutationY969F,S301Available SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
GPR25-DuET
Plasmid#213277PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rBrokenheart
Plasmid#203394PurposeAAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.DepositorInsertBrokenheart
UseAAVExpressionMammalianPromoterEF1aAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B15
Plasmid#135513PurposeMammalian expression of myc-tagged HLA-B*15:01DepositorInsertHLA-B*15:01 (HLA-B Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
nLYN267-gs10-NLS-VPR
Plasmid#188265PurposePlasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnLYN267 (LYN Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only