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pBMN_HA-TBK1 (kinase dead - D135N)

Plasmid

#199206

Purpose

Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

D135N

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon9 E545K

Plasmid

#16642

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA E545K (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Mutation

E545K; see note below

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pLKO-RB1-shRNA19

Plasmid

#25640

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 19 (RB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5c-Myc

Plasmid

#186616

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform C fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5c (KIF5C Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-Flag-Cdk9-IRES-eGFP

Plasmid

#28096

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pGM9

Plasmid

#220167

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

FUNDC1 (FUNDC1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-Kif5c

Plasmid

#71853

Purpose

mammalian expression of GFP-Kif5c

Depositor

Michelle Peckham

Article

Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

Insert

Kif5c (Kif5c Rat)

Tags

EGFP

Expression

Mammalian

Mutation

Kif5c starts at AA3

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-SOCS1

Plasmid

#129514

Purpose

Retroviral vector for the expression of human SOCS1 with a FLAG tag in N-terminal

Depositor

Gerardo Ferbeyre

Article

Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

Insert

SOCS1 (SOCS1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab4a-c-Myc

Plasmid

#79799

Purpose

Express TagBFP labeled human Rab4a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab4a (RAB4A Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pAcGP67A-mTLR4ecd-VLR

Plasmid

#187877

Purpose

Baculovirus protein expression of mouse TLR4 (residues 26–544) fused with hagfish variable lymphocyte receptor (VLR) (residues 126–200)

Depositor

Bruce Beutler

Article

Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

TLR4ecd (Tlr4 Mouse)

Expression

Insect

Mutation

includes residues 26–544 of mouse TLR4

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGGD_MiniTurboID-YFP

Plasmid

#222434

Purpose

Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.

Depositor

Dominique Bergmann

Article

Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

Insert

MiniTurboID (BirA mutant)

Use

Golden gate / green gate compatible cloning vector

Tags

Linker and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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IRES-GFP FoxG1

Plasmid

#14935

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxG1 (FOXG1 Human)

Tags

IRES-GFP

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3-Ctgf-rDMR

Plasmid

#235696

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Ctgf with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Ctgf (Ccn2 Mouse)

Use

Luciferase

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Ctgf-rDMRdeltaTEAD

Plasmid

#235697

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Ctgf lacking Tead-binding motif with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Ctgf (Ccn2 Mouse)

Use

Luciferase

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Flag-cMyc T58A

Plasmid

#20076

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

cMyc (MYC Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T58A

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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TFORF1883

Plasmid

#142882

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SOX30 (SOX30 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2

Plasmid

#221719

Purpose

Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-hTBK1-E696K-EGFP

Plasmid

#221554

Purpose

Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Mutation

Changed glutamate 696 to lysine

Promoter

EF1a

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PM-RasAR

Plasmid

#205569

Purpose

Plasma membrane-targeted RasAR.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

PM-RasAR (RAF1 Human, Synthetic)

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1

Plasmid

#187830

Purpose

Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST and TEV-mEGFP-GG Linker

Expression

Bacterial and Insect

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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