164,782 results

1. pUb PspCas13b + PspCas13b guide RNA

   Plasmid

   #176305

   Purpose

   Expresses PspCas13b and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   PspCas13b
   

   Use

   Tags

   HA tag

   Expression

   Insect

   Mutation

   E113K, N265S- please depositor comments

   Promoter

   Ubi-p63e

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-SPI1

   Plasmid

   #162824

   Purpose

   Doxycycline-inducible overexpression of human SPI1

   Depositor

   Matthew Steinhauser

   Article

   Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet ON

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB18cmBFPAzurite

   Plasmid

   #14034

   Depositor

   Patrick Daugherty

   Article

   Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

   Insert

   Azurite
   

   Use

   Yeast/bacteria optimized codons

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-3xHA-Puro

   Plasmid

   #207729

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

   Plasmid

   #67976

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AmAG cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-C-TEV-Twin-Strep

   Plasmid

   #202527

   Purpose

   Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMG-AIS

   Plasmid

   #225133

   Purpose

   For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.

   Depositor

   Lars Blank

   Article

   Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. qTAG-C-mScarlet-Puro-H2BC11

   Plasmid

   #207761

   Purpose

   Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLAS2w.Phyg-HAVCR1

   Plasmid

   #232669

   Purpose

   expresses HAVCR1 in mammalian cells

   Depositor

   Chen-Yang Shen

   Insert

   HAVCR1 (HAVCR1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212829

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Use

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG, TetOn

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJJB308

    Plasmid

    #107699

    Purpose

    AtU6 Promoter-LwaCas13a-gRNA Scaffold

    Depositor

    Daniel Voytas

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-mEGFP-CREB

    Plasmid

    #137001

    Purpose

    mEGFP-CREB donor for G-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-hM3D(Gq)-mCherry (AAV8)

    Viral Prep

    #50474-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    mCherry

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3.1_3XFlag

    Plasmid

    #182494

    Purpose

    Empty plasmid containing Flag tag for C-terminal tagging

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

    Insert

    3xFlag with 5 Glycolic spacer
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213969

    Purpose

    AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX4T-1_WT_OCT4

    Plasmid

    #40633

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV cleavage, and Thrombin cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBUN411

    Plasmid

    #50581

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    maize codon optimized

    Promoter

    OsU3p and Ubi1p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-C1-MKLP1

    Plasmid

    #70154

    Purpose

    mammalian expression of MKLP1 with an mCherry fusion

    Depositor

    Masanori Mishima

    Article

    Joseph et al EMBO J. 2012 May 30;31(11):2604-14. doi:

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1-sh-hSOX9-1

    Plasmid

    #40644

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-hSOX9-1 (SOX9 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ABE8e

    Plasmid

    #138489

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mPlum-C1

    Plasmid

    #54839

    Purpose

    Localization: C1 Cloning Vector, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pC-GoldyTALEN

    Plasmid

    #38143

    Purpose

    destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

    Depositor

    Daniel Carlson

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Tags

    AcV5 and FokI homodimer

    Expression

    Mammalian

    Mutation

    Truncate at N and C terminous

    Promoter

    miniCaggs

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSBbi-Pur

    Plasmid

    #60523

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGJJ045

    Plasmid

    #183621

    Purpose

    abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lenti_MS2_P65_HSF1_2A_puro

    Plasmid

    #118699

    Purpose

    To express MS2-P65-HSF1

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTO128_pPZP-NATca

    Plasmid

    #171105

    Purpose

    A. tumefaciens TI plasmid encoding NATca in T-DNA

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    NAT (Nourseothricin Resistance)
    

    Use

    A. tumefaciens ti plasmid

    Tags

    Expression

    Mutation

    Promoter

    AgTEF2

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid

    #165450

    Purpose

    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and short human rhodopsin promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-RHO-IntC-dCas9C-VPR-polyA

    Plasmid

    #166692

    Purpose

    Express C-terminal part of split dCas9-VPR

    Depositor

    Stylianos Michalakis

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Insert

    IntC-dCas9C-VPR
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    short human rhodopsin promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MBP-TSF-TEVcs-ULK1

    Plasmid

    #171416

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ULK1 (ULK1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CCR4-Tango

    Plasmid

    #66241

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CCR4 (CCR4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTW160C

    Plasmid

    #194801

    Purpose

    PCadBA3 expressing LuxAB

    Depositor

    Tina Wang

    Article

    Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

    Insert

    LuxAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBL069

    Plasmid

    #194800

    Purpose

    cCadC-GCN4 under the control of Ptet

    Depositor

    Tina Wang

    Article

    Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

    Insert

    cCadC-GCN4
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBL069B

    Plasmid

    #194802

    Purpose

    cCadC-GCN4* under the control of Ptet

    Depositor

    Tina Wang

    Article

    Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

    Insert

    cCadC-GCN4*
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. RCIscript-GoldyTALEN

    Plasmid

    #38142

    Purpose

    destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

    Depositor

    Daniel Carlson, Stephen Ekker

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Mrna transcription vector

    Tags

    AcV5 and FokI homodimer

    Expression

    Mutation

    Truncate at N and C terminous

    Promoter

    T3

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti_CMV:Flag-Antares

    Plasmid

    #208852

    Purpose

    Expresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    Antares
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. qTAG-C-V5-Puro

    Plasmid

    #207730

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pC0090

    Plasmid

    #181954

    Purpose

    Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    7xTOPFlash
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CMV-mCherry-KIX-mCherry

    Plasmid

    #137004

    Purpose

    mCherry-KIX acceptor for G-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    KIX domain (Crebbp Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. peGFP C3-SPG20 8xV

    Plasmid

    #218579

    Purpose

    Expresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Tryptophan 419, Leucine 438, Isoleucine 4…

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX

    Plasmid

    #208697

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge