We narrowed to 13,947 results for: crispr grnas

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pX330A-1x7

Plasmid

#58771

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_cmlc2(myl7)_cmlc2-nmKate

Plasmid

#238374

Purpose

Drives expression of 3 different gRNAs targeting cmlc2 (myl7), and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting cmlc2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p201B Cas9

Plasmid

#59177

Purpose

Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
BAR

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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Oct4-Cr1

Plasmid

#66989

Purpose

Expresses gRNA that targets stop codon for OCT4

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

gRNA for Crispr targeting the OCT4 stop codon

Expression

Mammalian

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Membrane proteins

Pooled Library

#1000000124

Purpose

CRISPR gRNA mouse knockout pooled library - Membrane proteins

Depositor

Michael Bassik

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72667

Purpose

Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDIRECT_10E

Plasmid

#91209

Purpose

Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLdCN

Plasmid

#84290

Purpose

Express gRNA and Cas9 in Leishmania with Neomycin resistance

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Promoter

L. donovani ribosome RNA promoter

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA

Pooled Library

#1000000095

Purpose

CRISPR gRNA knockout pooled library for targeting the human genome

Depositor

Eric Lander, David Sabatini

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
pMZ288

Plasmid

#59897

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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Bassik lab Mouse CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases

Pooled Library

#1000000122

Purpose

CRISPR gRNA mouse knockout pooled library - Drug targets, kinases, phosphatases

Depositor

Michael Bassik

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
pTol1-U6abc_ect2_cmlc2-nmKate

Plasmid

#238410

Purpose

Drives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting ect2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_tnnt2a_cmlc2-nmKate

Plasmid

#238309

Purpose

Drives expression of 3 different gRNAs targeting tnnt2a, and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_erbb2_cmlc2-nmKate

Plasmid

#238409

Purpose

Drives expression of 3 different gRNAs targeting erbb2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pBW1997_pExpr-hU6-CRISPR-DECODER

Plasmid

#87549

Purpose

Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

gRNAs targeting NGN2, MIAT, ACTC1, TTN

Use

CRISPR, Cre/Lox, and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1

Pooled Library

#1000000100

Purpose

CRISPR gRNA knockout pooled library (version 1) for targeting the human genome

Depositor

Eric Lander, David Sabatini

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
Sabatini/Lander Lab Activity-Optimized Genome-wide Mouse sgRNA Library in pLenti-sgRNA

Pooled Library

#1000000096

Purpose

CRISPR gRNA knockout pooled library for targeting the mouse genome

Depositor

Eric Lander, David Sabatini

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050

Pooled Library

#1000000131

Purpose

Genome-wide CRISPR gRNA pooled library for inhibition of mouse genes

Depositor

John Doench, David Root

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only
pLenti spCas9 T2A mNeonGreen P2A puro

Plasmid

#122183

Purpose

The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

Depositor

Raphael Gaudin

Inserts

spCas9 (NEWENTRY )
mNeonGreen

Use

Lentiviral

Tags

T2A

Expression

Mammalian

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

Plasmid

#60228

Purpose

Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA

Expression

Mammalian

Promoter

CBh and U6

Available Since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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