We narrowed to 5,278 results for: pcdna3.1

1. pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP

   Plasmid

   #67299

   Purpose

   Blue light dependent photoswitching system

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   Tiam1-pMagFast2(3x)-iRFP (Tiam1 Mouse, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+) Drp1 V2 S579A

   Plasmid

   #46342

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   Drp1 V2 (Dnm1l Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S579A

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1(+)Gαi2-91-NLuc

   Plasmid

   #207149

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAI2 (GNAI2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)Q

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-myc-AP1S3

   Plasmid

   #58292

   Purpose

   mammalian expression of AP1S3

   Depositor

   Francesca Capon

   Article

   Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

   Insert

   AP1S3 (AP1S3 Human)
   

   Use

   Tags

   6xHis and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1(+)-Mito-CaNARi

   Plasmid

   #64851

   Purpose

   Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean, N-terminal 30 amino acids from DAKAP1, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1_PA-Cre-Y324F

   Plasmid

   #122961

   Purpose

   Catalytically inactive mutant of PA-Cre

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

   Insert

   pcDNA3.1_PA-Cre-Y324F
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-MS2-HA-HsMex3C_AD

   Plasmid

   #148481

   Purpose

   Mammalian Expression of HsMex3C

   Depositor

   Elisa Izaurralde

   Insert

   HsMex3C (MEX3C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L11P in Mex3C

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 (-) hYAP 1-1delta

   Plasmid

   #124142

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Marius Sudol, Megan Finch-Edmondson

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   None (Wild Type)

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-MS2-HA-HshnRNPH1_Z

   Plasmid

   #148120

   Purpose

   Mammalian Expression of HshnRNPH1

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPH1 (HNRNPH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-CFP-KRAS4B

    Plasmid

    #112717

    Purpose

    Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    CFP-KRAS4B (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 FLIPE-100u

    Plasmid

    #13543

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    S118W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1(−) mCER-Prx2

    Plasmid

    #158224

    Purpose

    Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    mCER-Prx2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-sapphireAKAR-NLS

    Plasmid

    #118467

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR-NLS
    

    Use

    Tags

    Nuclear localization signal (NLS)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-RaACR_687-EYFP

    Plasmid

    #111077

    Purpose

    Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RaACR_687
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1his p66shc S36A

    Plasmid

    #10973

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

    Insert

    p66shc (SHC1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    S36A

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-dCas9-CibN

    Plasmid

    #60552

    Purpose

    Expresses dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1/HisC 14-3-3 epsilon wt

    Plasmid

    #11942

    Depositor

    Michael Yaffe

    Article

    Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

    Insert

    Ywhae (Ywhae Rat)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1 FLIPglu-3.2mDelta13V

    Plasmid

    #18011

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu F16A S112A Delta13
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    F16A, S112A in binding protein

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(-) Hu 4-1BB(CD137)-Fc

    Plasmid

    #183251

    Purpose

    Soluble expression of human 4-1BB (CD137)-Fc. Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Insert

    41BB Fc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1_mGFP-FGFR3-K650E

    Plasmid

    #191757

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1948A>G (p.K650E) + silent mutation c.1938C&…

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge