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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,123 results for: multi

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Showing: 9181 - 9200 of 18123 results

  3. Ad:2CMV_A2R2-N25K3

    Plasmid
    #122242
    Purpose
    Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Corey Neu
    Article
    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516
    Inserts
    C-terminal end of Nesprin-3 (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    122242-plasmid-map-sequence-id-238909

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  7. Mouse 5' Npm1 AID GFP PuroR

    Plasmid
    #127899
    Purpose
    HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Npm1 Gene
    Depositor
    Mark Groudine
    Insert
    Npm1 (Npm1 Mustard Weed, Mouse)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127899-plasmid-map-sequence-id-250993

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  9. Gal4-entry+NLS

    Plasmid
    #128013
    Purpose
    Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer
    Depositor
    Julius Brennecke
    Article
    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-
    Insert
    Gal4 DBD-SV40NLS-3x-Flag
    Use
    Gal4 entry vector with nls to generate tethering …
    Tags
    Gal4-DBD-SV40NLS-3xFlag
    Available Since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128013-plasmid-map-sequence-id-251575

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  10. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Insert
    F5-flanked tetracycline resistance cassette
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Available Since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  13. pYZ172

    Plasmid
    #98409
    Purpose
    Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.
    Depositor
    Jef Boeke
    Article
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Inserts
    upstream homologous region to delete lys9 in genome by recombination
    downstream homologous region to delete lys9 in genome by recombination
    Use
    CRISPR and Synthetic Biology
    Available Since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98409-plasmid-map-sequence-id-198900

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  14. pJK372

    Plasmid
    #73597
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73597-plasmid-map-sequence-id-138754

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  16. p221- purVSG117UTR

    Plasmid
    #59732
    Purpose
    Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.
    Depositor
    Gloria Rudenko
    Article
    Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:
    Insert
    VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
    Use
    For homologous recombination into vsg221 expressi…
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    59732-plasmid-map-sequence-id-100249

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  17. p148

    Plasmid
    #62616
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62616-plasmid-map-sequence-id-102932

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  18. pTSlb-R756K

    Plasmid
    #60731
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available Since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60731-plasmid-map-sequence-id-99358

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Showing: 9181 - 9200 of 18123 results