We narrowed to 19,051 results for: Tec

1. Polymerase expression - pCMV-ePhi29 (LM2802)

   Plasmid

   #208967

   Purpose

   Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGK1 gRNA (BRDN0001146969)

   Plasmid

   #77982

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PGK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PGK1 (PGK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRIM28 gRNA (BRDN0001162440)

   Plasmid

   #76140

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM28

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pv9_CBX7_Chromo/TAF3.PHD-13XL-BASU

   Plasmid

   #179415

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.Chromo/TAF3.PHD-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7.Chromo/TAF3.PHD-13XL-BASU
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. STK4 gRNA (BRDN0001147987)

   Plasmid

   #76075

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK4 (STK4 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut

   Plasmid

   #187154

   Purpose

   mammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PFKFB3 gRNA (BRDN0001147151)

   Plasmid

   #76461

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PFKFB3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PFKFB3 (PFKFB3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PFKFB3 gRNA (BRDN0001147212)

   Plasmid

   #76463

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PFKFB3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PFKFB3 (PFKFB3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-MBP-(CBX1)3-WT

   Plasmid

   #180212

   Purpose

   Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1 x3 (CBX1 Human)
   

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmKO-imp α

    Plasmid

    #200082

    Purpose

    Expresses mKO-tagged human importinα in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

    Insert

    importin α (KPNA2 Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCALNL-mutKir2.1

    Plasmid

    #125579

    Purpose

    Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A non-conducting mutant (GYG -> AAA).

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pINTO-NSA::mEzh2

    Plasmid

    #61725

    Purpose

    Expression of mouse Ezh2 fused to monomeric streptavidin (N-terminus)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Insert

    Enhancer of zeste homolog 2 (Ezh2 Mouse)
    

    Tags

    mSA (monomeric streptavidin)

    Expression

    Mammalian

    Promoter

    T-REx (CMV + 2xTetO2)

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. SECURE BE4max(R33A) (pBM608)

    Plasmid

    #140251

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. STK4 gRNA (BRDN0001149002)

    Plasmid

    #76074

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK4 (STK4 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. PIKFYVE gRNA (BRDN0001144909)

    Plasmid

    #76890

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIKFYVE (PIKFYVE Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid

    #208969

    Purpose

    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PRKAA1 gRNA (BRDN0001146526)

    Plasmid

    #76253

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAA1 (PRKAA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-BE3RA-P2A-GFP-PGK-Puro

    Plasmid

    #110868

    Purpose

    Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

    Plasmid

    #160571

    Purpose

    Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNAscaffold 2.1 scRNA
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123338

    Purpose

    Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    DAKAP-Venus-cpVenus-FLARE-AKAR
    

    Tags

    N-terminal 30 amino acids from DAKAP1, Venus, and…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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