We narrowed to 22,932 results for: tes

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XXXXA_Q182A_R287H-pHG165c

Plasmid

#170111

Purpose

Constitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA_Q182A_R278H

Expression

Bacterial

Mutation

Q182A, R287H

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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RRRAA-pHG165c

Plasmid

#170112

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

RRRAA

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin-TAPBPR 22-35

Plasmid

#153478

Purpose

Mammalian expression of FLAG-tagged Tapasin with TAPBPR a.a. 22-35

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

Tapasin a.a.10-20 replaced with TAPBPR a.a. 22-35

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin delta2step

Plasmid

#153479

Purpose

Mammalian expression of FLAG-tagged Tapasin delta2step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.13-14, 16-17

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pCEP4-FLAG-Tapasin delta3step

Plasmid

#153480

Purpose

Mammalian expression of FLAG-tagged Tapasin delta3step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.12-14, 16-18

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pCEP4-FLAG-Tapasin G15E

Plasmid

#153481

Purpose

Mammalian expression of FLAG-tagged Tapasin G15E

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

G15E

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin G15L

Plasmid

#153482

Purpose

Mammalian expression of FLAG-tagged Tapasin G15L

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

G15L

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin L18E

Plasmid

#153483

Purpose

Mammalian expression of FLAG-tagged Tapasin L18E

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

L18E

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin L18G

Plasmid

#153484

Purpose

Mammalian expression of FLAG-tagged Tapasin L18G

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

L18G

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin L18K

Plasmid

#153485

Purpose

Mammalian expression of FLAG-tagged Tapasin L18K

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

L18K

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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SaLgCG

Plasmid

#164563

Purpose

GFP

Depositor

Junwei Shi

Article

Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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MPC026: pAAV-CAG-FLEX-NovArch-TS-Citrine_TSx3-ER2

Plasmid

#153195

Purpose

encodes pAAV NovArch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

NovArch

Use

AAV

Tags

citrine

Expression

Mammalian

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TAB-mOvol1

Plasmid

#120360

Purpose

piggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

Ovol1 (Ovol1 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tet-on and CBh

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-3

Plasmid

#160703

Purpose

tef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tef1-alpha

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 R14A/K310A

Plasmid

#162655

Purpose

Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acids R14 and K310 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-ORF68 C52A

Plasmid

#162660

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C52 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

Plasmid

#162639

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids K174, R179, and K182 mutated to alani…

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 K435A

Plasmid

#162651

Purpose

Expresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acid K435 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 K450A/R451A

Plasmid

#162653

Purpose

Expresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acids K450 and R451 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27-H112A RNAi-resistant

Plasmid

#163621

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-H112A RNAi-resistant (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

H112A and RNAi resistance

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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