231,910 results

1. Flag-GFP-HRP-TM_pLX304

   Plasmid

   #202021

   Purpose

   expresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   Flag-GFP-HRP-TM
   

   Use

   Lentiviral

   Tags

   Flag and GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

   Plasmid

   #196677

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   PKIα-P2A-mRuby3
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGK-HALO-NTERM-GFP

   Plasmid

   #185772

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, Halo

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Rab7A Q67L

   Plasmid

   #28049

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab7A (RAB7A Human)
   

   Tags

   2xHA and EGFP

   Expression

   Mammalian

   Mutation

   Q67L

   Available Since

   May 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-Rab7A Q67L

   Plasmid

   #28049

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab7A (RAB7A Human)
   

   Tags

   2xHA and EGFP

   Expression

   Mammalian

   Mutation

   Q67L

   Available Since

   May 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAurora2

   Plasmid

   #213134

   Purpose

   Integrated transcriptional and translational control for stringently blue-light-activated gene expression

   Depositor

   Andreas Moeglich

   Article

   Ranzani et al Nucleic Acids Res. 2024 Aug 10:gkae678.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-SLC38A10_STOP

   Plasmid

   #161249

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC38A10 (SLC38A10 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pMBP-FnCas12a

   Plasmid

   #113432

   Purpose

   Expresses 10xHis-MBP fused to FnCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   FnCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-Opto-Acvr1ba-P2A-Acvr2ba

   Plasmid

   #232640

   Purpose

   Optogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expression

   Depositor

   Katherine Rogers

   Insert

   Acvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
   

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-CMV-PdCO-mScarlet

    Plasmid

    #198507

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LXRE_Luc

    Plasmid

    #177622

    Purpose

    synthetic 3X LXRE promoter linked to luciferase in the pTAL vector

    Depositor

    Thomas Burris

    Article

    Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

    Insert

    3X LXRE
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDZ2087

    Plasmid

    #92414

    Purpose

    Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

    Insert

    TEV protease
    

    Tags

    MBP (self cleaving)

    Expression

    Bacterial

    Mutation

    L56V/S135G/S219V

    Promoter

    tac

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDZ2087

    Plasmid

    #92414

    Purpose

    Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

    Insert

    TEV protease
    

    Tags

    MBP (self cleaving)

    Expression

    Bacterial

    Mutation

    L56V/S135G/S219V

    Promoter

    tac

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xnls-mScarlet-I

    Plasmid

    #98816

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    3xNLS
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xnls-mScarlet-I

    Plasmid

    #98816

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    3xNLS
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUW-tetO-NFKB1

    Plasmid

    #139822

    Purpose

    doxycycline-inducible expression of Human NFKB1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    NFKB1 (NFKB1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV9)

    Viral Prep

    #83896-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    hDlx

    Tags

    dTomato (physically separate, not a fusion protein)

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

18. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid

    #206042

    Purpose

    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    

    Use

    CRISPR and Synthetic Biology; Donor template

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-Peredox

    Plasmid

    #73807

    Purpose

    Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)

    Depositor

    Gary Yellen

    Article

    Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

    Insert

    Peredox
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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