We narrowed to 23,679 results for: crispr

1. pGEL675

   Plasmid

   #196822

   Purpose

   dLrCas9 gene expression repression plasmid in rice

   Depositor

   Yong Zhang

   Article

   Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   ZmUbi

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEL677

   Plasmid

   #196824

   Purpose

   LrCas9 gene expression activation plasmid in rice

   Depositor

   Yong Zhang

   Article

   Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   ZmUbi

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU1/Sm/3' Box_(GLuc)_INT

   Plasmid

   #68438

   Purpose

   Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three PP7 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U1

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. JEH127

   Plasmid

   #179299

   Purpose

   Expresses dCas9 fused to VPR

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

   Insert

   dCas9-VPR
   

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   CAG

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-GSX2-tTA-BleoR

   Plasmid

   #161748

   Purpose

   Tet-ON tTA plasmid for the c-terminal targeting of human GSX2 locus

   Depositor

   Elena Cattaneo

   Article

   Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

   Insert

   Advanced tTA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   endogenous GSX2 promoter

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbdCas9S(-10AC12)_Psyn-sgRNArodA

   Plasmid

   #149661

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi rodA

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNArodA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30(-10AC12), Psyn

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLib6.4B

   Plasmid

   #176665

   Purpose

   Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

   Plasmid

   #149588

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi flaB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAflaB
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, Psyn

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6_(GLuc)_INT[P4-P6(3xPP7)]

   Plasmid

   #68432

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS24: pCascade(PmcCAST)_entry

    Plasmid

    #168157

    Purpose

    Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST minimal CRISPR array
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJZC593

    Plasmid

    #62319

    Purpose

    sgRNA with MS2-PP7 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + MS2-PP7 RNA binding module
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

    Plasmid

    #149590

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAftsI
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLib6.6B

    Plasmid

    #176666

    Purpose

    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJZC572

    Plasmid

    #62318

    Purpose

    sgRNA with 1x com for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x com RNA binding module
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop

    Plasmid

    #169369

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CPIJ039653

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDECKO_Malat1_E

    Plasmid

    #72624

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Expression

    Mammalian

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. SGL40C.EFS.RFP657

    Plasmid

    #69147

    Purpose

    Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-AaCas12b(R911A)-2AeGFP

    Plasmid

    #121961

    Purpose

    Mammalian expression, Transcription regulation, deactivated AaCas12b

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    Catalytically deactivated AaCas12b
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    R911A

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiU6-mNeurog2 gRNA_1-MS2-Puro

    Plasmid

    #192680

    Purpose

    Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ141-ZmUbi-RZ-Aa3.8.5

    Plasmid

    #158407

    Purpose

    Express single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Maize ubiquitin 1

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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