We narrowed to 19,051 results for: Tec

1. pLenti-HF1RA-P2A-Puro

   Plasmid

   #110853

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PRKD1 gRNA (BRDN0001146537)

   Plasmid

   #77228

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKD1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKD1 (PRKD1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192788

   Purpose

   aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody enhancer
   

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MLM3713

   Plasmid

   #49946

   Purpose

   Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x FLAG Tet1CD RH-4 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Promoter

   EF1a

   Available Since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

5. PFKFB3 gRNA (BRDN0001146230)

   Plasmid

   #76462

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PFKFB3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PFKFB3 (PFKFB3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRB144

   Plasmid

   #180175

   Purpose

   pCDNA3.1 Construct GluN1 A714L

   Depositor

   Richard Bergeron

   Article

   Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

   Insert

   GluN1 (Grin1 Rat)
   

   Tags

   Phluorin

   Expression

   Mammalian

   Mutation

   A714L

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. OSR1 gRNA (BRDN0001146927)

   Plasmid

   #76147

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human OSR1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   OSR1 (OSR1, OXSR1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. OSR1 gRNA (BRDN0001145611)

   Plasmid

   #76148

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human OSR1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   OSR1 (OSR1, OXSR1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. OSR1 gRNA (BRDN0001145425)

   Plasmid

   #76149

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human OSR1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   OSR1 (OSR1, OXSR1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PI4K2A gRNA (BRDN0001146485)

    Plasmid

    #76820

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PI4K2A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PI4K2A (PI4K2A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PI4K2A gRNA (BRDN0001146736)

    Plasmid

    #76821

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PI4K2A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PI4K2A (PI4K2A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. SIK2 gRNA (BRDN0001148975)

    Plasmid

    #77139

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SIK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SIK2 (SIK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PRKAR1A gRNA (BRDN0001146329)

    Plasmid

    #77720

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1A (PRKAR1A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. NADK2 gRNA (BRDN0001147240)

    Plasmid

    #78092

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NADK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NADK2 (NADK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PET-SpCas9-HF1-plus-NLS-6xHis

    Plasmid

    #207381

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, Q695A, Q926A, amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-B-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207385

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MLM3762

    Plasmid

    #49947

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x FLAG Tet1CDmut RH-4 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX

    Plasmid

    #163571

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTOR gRNA (BRDN0001145868)

    Plasmid

    #76784

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MTOR

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MTOR (MTOR Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PDGFRB gRNA (BRDN0001147806)

    Plasmid

    #77059

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PDGFRB

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge