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pLEX-EF1a ANXA11-mEmerald R235Q

Plasmid

#164212

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

R235Q

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag ILK

Plasmid

#20505

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ILK (ILK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDNA5-FRT/TO-FLAG-TONSL

Plasmid

#221011

Purpose

mammalian expression vector of Flag tagged TONSL siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

TONSL (TONSL Human)

Tags

Flag

Expression

Mammalian

Mutation

silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

Promoter

CMV

Available Since

June 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV_hSyn-PdCO-EGFP-WPRE

Plasmid

#198513

Purpose

Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAP3K7

Plasmid

#20520

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP3K7 (MAP3K7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Q356H

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pDCAF3-IS5

Plasmid

#65220

Purpose

Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

Depositor

Jeffrey Barrick

Article

Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

Inserts

ndmA
ndmB
ndmC
ndmD
gst9
chlR

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed start codon from gtg to atg and Changed s…

Promoter

BBa_J23100

Available Since

Jan. 8, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-FGFR3

Plasmid

#23933

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGFR3 (FGFR3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE_FKBP-mEGFP-WIPI2d_P2A-FRB-Fis1 (no mt-mKeima)

Plasmid

#223794

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

2xFKBP-mEGFP-WIPI1-(P2A)-FRB-Fis1 (WIPI1, FIS1 Human)

Use

Lentiviral

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human PP2A B56α full length

Plasmid

#132635

Purpose

expresses human PP2A B56α full length in E. coli

Depositor

Wenqing Xu

Article

Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

Insert

PP2A B56α

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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AiP981-pAAV-mscRE4-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163484

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP981 - pAAV-AiE2004m-minBG-EGFP-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-SIO-PdCO-mScarlet-WPRE

Plasmid

#198509

Purpose

Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11(R235Q)

Plasmid

#164205

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 R235R mutant under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

R235Q

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

Plasmid

#198515

Purpose

Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

EF-1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBFC1207

Plasmid

#186694

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag ITK

Plasmid

#20507

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ITK (ITK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE3 (minus strand)

Plasmid

#91845

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0622)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE3 (minus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Missing 1 base from 13 base polyT tract beginning…

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11(D40G)

Plasmid

#164204

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 D40G mutant under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

D40G

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag LCK

Plasmid

#20511

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

LCK (LCK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport

Plasmid

#129180

Purpose

Fluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Use

AAV

Tags

Ig-kappa leader, Myc, and PDGFR + enhanced ER exp…

Promoter

CAG

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDmelOR-mRho.V5.mER.hOr56a

Plasmid

#126479

Purpose

Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Inserts

Or56a (Or56a Fly)
Orco (Orco Fly)

Tags

H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

Expression

Mammalian

Mutation

codon optimization for H. sapiens and codon optim…

Promoter

CMV

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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