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pFA6a-Pho8∆60-His3MX6

Plasmid

#240971

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HIS3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-empty

Plasmid

#239099

Purpose

pGL3 luciferase vector with no promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Type

Empty backbone

Use

Luciferase

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-C-EF1α

Plasmid

#239097

Purpose

Empty pcDNA4-C backbone with EF1α promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Type

Empty backbone

Expression

Mammalian

Promoter

EF1α

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLP-PrhaBAD-pcepA-himar-chlorR

Plasmid

#238492

Purpose

Landing Pad with RhaRS/PrhaBAD, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

RhaRS/PrhaBAD

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP64 (pAVA3500)

Plasmid

#239237

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP64

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP24 (pAVA3547)

Plasmid

#239240

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP24 (pAVA3555)

Plasmid

#239242

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP24 (pAVA3563)

Plasmid

#239258

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP64 (pAVA3572)

Plasmid

#239259

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP64

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LSAM domain expression plasmid

Plasmid

#240215

Purpose

Plasmid for the recombinant expression of the LSAM domain of human legumain in E. coli.

Depositor

Elfriede Dall

Article

Dahms et al STAR Protocols, 6, 3, 103991, 19 Septemb

Insert

LSAM (LGMN Human)

Tags

PC1pro

Expression

Bacterial

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-NanA-CSS-ET64

Plasmid

#234666

Purpose

Expression an ELP-tagged fusion enzyme of NanA and CSS

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

sialic acid aldolase
CMP-sialic acid synthetase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-ManC-NahK-ET64

Plasmid

#234664

Purpose

Expression an ELP-tagged fusion enzyme of PfManC and NahK

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

GDP-Man pyrophosphorylase
N-acetylhexosamine 1-kinase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPath-pcepA-integrase vector-v2

Plasmid

#238495

Purpose

Entry vector for biosynthetic pathway, pcepA-phiC31 integrase, altered to be compatible with inducible landing pads generated in this study

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-KO-454TAG

Plasmid

#240063

Purpose

Express AAV2-KO (R585,588A) capsid with ncAA mutant at site 454

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Insert

AAV2-454-TAG, R585,588A

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-454TAG

Plasmid

#240058

Purpose

Express AAV2 capsid with ncAA mutant at site 454

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Insert

AAV2 Rep Cap-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF_AaRaf1/BSD2

Plasmid

#229516

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,BSD2

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
BSD2

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLP-PH16_RS09790-pcepA-himar-chlorR

Plasmid

#238494

Purpose

Landing Pad with BenM/PH16_RS09790, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

BenM/PH16_RS09790

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLP-ParaBAD-pcepA-himar-chlorR

Plasmid

#238491

Purpose

Landing Pad with AraC/ParaBAD, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

AraC/ParaBAD

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLP-PH16_RS08125-pcepA-himar-chlorR

Plasmid

#238493

Purpose

Landing Pad with NahR/PH16_RS08125, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

NahR/PH16_RS08125

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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P-d22bp

Plasmid

#235657

Purpose

B. thetaiotaomicron fusA2 expression reporter lacking 22bp Cur binding site

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides thetaiotaomicron fusA2 promoter lacking the 22bp Cur binding site

Mutation

The B. thetaiotaomicron fusA2 promoter lacking &q…

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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