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TOPO CMV

Plasmid

#68454

Purpose

TOPO construct expressing CMV for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cytomegalovirus Immediate-Early Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pPV454

Plasmid

#42922

Depositor

James Lok

Article

Stoltzfus et al PLoS One. 2012;7(6):e38587. doi: 10.1371

Insert

Ce-age-1 promoter (873 bp)::Ce-age-1 cDNA (3549 bp)::Ce-unc-54 terminator (765 bp) (age-1 Nematode)

Expression

Worm

Promoter

Ce-age-1 promoter (873 bp)

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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SPIB_pet28a

Plasmid

#131655

Purpose

Bacterial expression of ETS gene SPIB

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPIB (SPIB Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBS YBX1

Plasmid

#19217

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

YBX1 (YBX1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pEE386

Plasmid

#149284

Purpose

T-DNA encoding TRV2 with gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEE388

Plasmid

#149286

Purpose

T-DNA encoding TRV2 with mFT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_mFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
pEE385

Plasmid

#149289

Purpose

T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPAG1_TPR

Plasmid

#109873

Purpose

Protein expression and purification of SPAG1_TPR

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SPAG1_TPR (SPAG1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDA125

Plasmid

#74249

Purpose

Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).

Depositor

Serge Pelet

Article

Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

Insert

Inducible peptide of the dPSTR

Expression

Bacterial and Yeast

Promoter

pSTL1

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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SUN2delSUN

Plasmid

#130835

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Tags

mCherry-FLAG-HA

Expression

Plant

Mutation

Deletion of SUN domain

Promoter

CaMV 35S

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSECB

Plasmid

#106305

Purpose

Express spCas9 with sgRNA, blast resistance

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;clec14aCE1-basEgfp

Plasmid

#90140

Purpose

contains clec14a enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aE1basEgfp

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_U2AF1_RRM

Plasmid

#110033

Purpose

Protein expression and purification of U2AF1_RRM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

U2AF1_RRM (U2AF1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NXT1_NTF2

Plasmid

#109803

Purpose

Protein expression and purification of NXT1_NTF2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NXT1_NTF2 (NXT1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab3aa

Plasmid

#109703

Purpose

Multisite gateway vector for 3' tagging with rab3aa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab3aa (rab3aa Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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RhoJ

Plasmid

#55696

Purpose

expression of mouse RhoJ

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoJ (Rhoj Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pPD1122 CMV 3xFLAG-NES-PYL1-VP64 in TUPV3

Plasmid

#161547

Purpose

Constitutive expression of 3xFLAG-NES-PYL1-VP64 under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NES-PYL1-VP64

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSG 413

Plasmid

#63859

Purpose

Detect translation initiation from CUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

CUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_VCL_Vinculin

Plasmid

#110045

Purpose

Protein expression and purification of VCL_Vinculin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VCL_Vinculin (VCL Human)

Expression

Bacterial

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFP.R365

Plasmid

#138235

Purpose

Expresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(HGK).EP-Pri*.(TEV)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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